Regulation of human hsp90α gene expression
Regulation of human hsp90α gene expression
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DOI：
10.1016/s0014-5793(99)00044-7
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发表时间：
1999-02
期刊：
影响因子：
3.5
通讯作者：
Shu-ling Zhang;Jun Yu;Xiao-kuan Cheng;Li Ding;F. Heng;N. Wu;Yu‐fei Shen
中科院分区：
文献类型：
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作者：
Shu-ling Zhang;Jun Yu;Xiao-kuan Cheng;Li Ding;F. Heng;N. Wu;Yu‐fei Shen
哺乳动物HSP 90 α和HSP 90 β由两个独立的基因编码。根据人hsp 90 α基因上游序列（GenBank登录号为U25822），构建了由hsp 90 α基因各片段驱动的CAT报告质粒。我们发现：（1）位于−96/−60的近端热休克元件复合物增强hsp 90 α启动子表达;（2）热休克诱导依赖于位于−1031/−1022的远端热休克元件和hsp 90 α基因的近端热休克元件复合物的共存;（3）与hsp 90 β不同，转录起始位点的下游序列抑制hsp 90 α表达。结论：hsp 90 α和hsp 90 β基因表达的调控机制不同。
Mammalian HSP90α and HSP90β are encoded by two individual genes. On the basis of the upstream sequences of the human hsp90α gene, GenBank accession number U25822, we have constructed CAT reporter plasmids driven by individual fragments of the hsp90α gene. We found that (1) the proximal heat shock element complex located at −96/−60 enhances hsp90α promoter expression; (2) heat shock induction depends upon the coexistence of distal heat shock element at −1031/−1022 and the proximal heat shock element complex of the hsp90α gene; (3) unlike hsp90β, downstream sequences of the transcription start site inhibit hsp90α expression. We conclude that the regulatory mechanisms for the expression of hsp90α and hsp90β genes are different.