Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron.
Good hydration and cell-biological performances of superparamagnetic calcium phosphate cement with concentration-dependent osteogenesis and angiogenesis induced by ferric iron.
复制标题
DOI：
10.1039/c5tb01440a
复制
发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Jing Zhang;Haishan Shi;Jingqun Liu;Tao Yu-;Zuguang Shen;Jiandong Ye
中科院分区：
文献类型：
--
作者：
Jing Zhang;Haishan Shi;Jingqun Liu;Tao Yu-;Zuguang Shen;Jiandong Ye
磷酸钙水泥（CPC）的多功能性可以通过不同金属离子的共掺杂来实现。磁力和热疗已被提议作为骨愈合和抗骨肉瘤治疗的潜在治疗方法。生物材料中的铁掺杂已被证实可以满足这些治疗的临床要求。在此，随着Fe3+浓度的增加，超顺磁性铁掺杂CPC（Fe-CPC）表现出改善的可注射性和抗压强度、增加的负表面电荷并加速水合作用。通过振动样品磁力计（VSM）分析证实了Fe-CPC的超顺磁性。在 Fe-CPC 盘上培养的小鼠骨髓基质细胞 (mBMSC) 表现出更好的附着形态和增殖，并且成骨相关基因表达增强。此外，在细胞培养基中浸出一系列不同浓度Fe3+的提取物，以模拟磁性生物材料周围含Fe3+的液体环境。在 Fe3+ 提取物中培养的 mBMSC 和人脐静脉内皮细胞 (HUVEC) 的性能显示，在一定量的 Fe3+ 下，增殖率增加。观察到Fe3+诱导的成骨和血管生成，但当Fe3+浓度超过临界值时，mBMSCs出现细胞毒性。 Fe-CPC通过结合原位自凝性、可注射性、超顺磁性、成骨作用、血管生成作用和骨传导性，有望在骨重塑中具有广阔的应用前景。
The multifunctionality of calcium phosphate cement (CPC) can be achieved via co-doping with different metallic ions. Magnetism and hyperthermia have been proposed as potential therapeutic methods in bone healing and anti-osteosarcoma treatment. Iron-doping in biomaterials has been confirmed to meet the clinical requirements for these treatments. Herein, superparamagnetic iron-doped CPC (Fe-CPC) showed improved injectability and compressive strength, increased negative surface charge and accelerated hydration with increasing Fe3+ concentration. The superparamagnetism of Fe-CPC was confirmed through vibrating sample magnetometer (VSM) analysis. Mouse bone marrow stromal cells (mBMSCs) cultured on Fe-CPC disks exhibited better attachment morphology and proliferation, and had an enhancement of osteogenic-related gene expression. Moreover, a series of extracts with different concentrations of Fe3+ in cell culture medium were leaching-prepared to simulate the Fe3+-containing liquid environment around the magnetic biomaterials. The performances of mBMSCs and human umbilical vein endothelial cells (HUVECs) cultured in Fe3+-extracts showed increased proliferation rate in a certain amount of Fe3+. Osteogenesis and angiogenesis induced by Fe3+ were observed, but cytotoxicity in mBMSCs appeared when the concentration of Fe3+ was beyond a critical value. Fe-CPC is supposed to have prospective applications in bone remodeling through the combination of self-setting in situ, injectability, superparamagnetism, osteogenesis, angiogenesis, and osteoconductivity.