Imbalances of CD4+ T-cell subgroups in Crohn's disease and their relationship with disease activity and prognosis
Imbalances of CD4+ T-cell subgroups in Crohn's disease and their relationship with disease activity and prognosis
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DOI：
10.1111/jgh.12592
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发表时间：
2014-10-01
影响因子：
4.1
通讯作者：
Chen, Minhu
中科院分区：
文献类型：
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作者：
Chao, Kang;Zhang, Shenghong;Chen, Minhu
背景和目的CD 4（+）T细胞亚群在克罗恩病（CD）中起着重要的病理生理作用;然而，它们的临床意义需要进一步澄清，并且仍然存在争议。方法收集46例CD患者和23例健康献血员外周血单个核细胞和血清，测定CD患者外周血单个核细胞和血清中CD 4+、CD 8+、CD流式细胞术分析循环Treg、Th 1、Th 2和Th 17细胞。亚组限制性转录因子的表达通过实时聚合酶链反应测定。通过流式细胞仪微球阵列或酶联免疫吸附试验测量每个亚组产生的主要细胞因子的血清浓度。（6.01.2% vs 7.8 ± 1.5%，P=0.030），FOXP 3 mRNA表达CD患者与对照组相比，循环可溶性TGF-1（19.1 +/- 9.9 vs 32.7 +/- 16.8ng/mL，P=0.038）。CD患者Th 1和Th 17细胞比例较高（17.8 ± 6.6% vs 7.8 ± 1.5%，P
Background and AimThe CD4(+) T-cell subgroups play central pathophysiological roles in Crohn's disease (CD); however, their clinical relevance requires additional clarification and remains controversial. We investigated their balance in Chinese CD patients and explored their clinical significance.MethodsPeripheral blood mononuclear cells and serum were collected from 46 Chinese CD patients and 23 healthy donors. Circulating Treg, Th1, Th2, and Th17 cells were flow cytometrically analyzed. Subgroup-restricted transcription factor expression was determined by real-time polymerase chain reaction. Serum concentrations of the main cytokines produced by each subgroup were measured by cytometric bead arrays or enzyme-linked immunosorbent assay.ResultsLower Treg proportion (6.01.2% vs 7.8 +/- 1.5%, P=0.030), FOXP3 mRNA expression (0.58-fold, P=0.030), and circulating soluble TGF-1 (19.1 +/- 9.9 vs 32.7 +/- 16.8ng/mL, P=0.038) were observed in CD patients versus controls. The Th1 and Th17 proportions were higher in CD patients (17.8 +/- 6.6% vs 7.8 +/- 1.5%, P