Evidence for the involvement of annexin 6 in the trafficking between the endocytic compartment and lysosomes
Evidence for the involvement of annexin 6 in the trafficking between the endocytic compartment and lysosomes
复制标题
DOI：
10.1006/excr.2001.5268
复制
发表时间：
2001-09-10
影响因子：
3.7
通讯作者：
Enrich, C
中科院分区：
文献类型：
--
作者：
Pons, M;Grewal, T;Enrich, C
膜联蛋白是一类钙离子依赖性磷脂结合蛋白，参与多种生物学过程，包括膜转运。用低密度脂蛋白（LDL）负载NRK细胞膜联蛋白6/lgp 120前溶酶体区室，然后研究其从该内吞区室的转运及其在溶酶体中的降解。用突变的膜联蛋白6（anx 6（1-175））显微注射NRK细胞，以评估膜联蛋白6可能参与LDL从前溶酶体区室的转运。结果表明，在NRK细胞中显微注射突变的膜联蛋白6，显示LDL在较大的内吞结构中积累，表示LDL保留在前溶酶体隔室中。为了证实膜联蛋白6参与LDL的运输和降解，我们使用了用突变的膜联蛋白6（1-175）转染的CHO细胞。因此，与NRK细胞一致，在CHO细胞中获得的结果表明，与过表达野生型膜联蛋白6的对照相比，表达突变形式的膜联蛋白6的CHO细胞中LDL降解受到显著抑制。因此，我们的结论是，一个连接蛋白6参与的贩运事件，导致低密度脂蛋白降解。(C)北京：科学出版社.
Annexins are a family of calcium-dependent phospholipid-binding proteins, which have been implicated in a variety of biological processes including membrane trafficking. The annexin 6/lgp120 prelysosomal compartment of NRK cells was loaded with low-density lipoprotein (LDL) and then its transport from this endocytic compartment and its degradation in lysosomes were studied. NRK cells were microinjected with the mutated annexin 6 (anx6(1-175)), to assess the possible involvement of annexin 6 in the transport of LDL from the prelysosomal compartment. The results indicated that microinjection of mutated annexin 6, in NRK cells, showed the accumulation of LDL in larger endocytic structures, denoting retention of LDL in the prelysosomal compartment. To confirm the involvement of annexin 6 in the trafficking and the degradation of LDL we used CHO cells transfected with mutated annexin 6(1-175). Thus, in agreement with NRK cells the results obtained in CHO cells demonstrated a significant inhibition of LDL degradation in CHO cells expressing the mutated form of annexin 6 compared to controls overexpressing wild-type annexin 6. Therefore, we conclude that an-nexin 6 is involved in the trafficking events leading to LDL degradation. (C) 2001 Academic Press.