Serotonin and serotonin transporter in the rectum of patients with irritable bowel disease
Serotonin and serotonin transporter in the rectum of patients with irritable bowel disease
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DOI：
10.3892/mmr.2013.1525
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发表时间：
2013-08-01
影响因子：
3.4
通讯作者：
Gundersen, Doris
中科院分区：
文献类型：
--
作者：
El-Salhy, Magdy;Wendelbo, Ingvild;Gundersen, Doris
肠易激综合征（IBS）是一种常见的慢性胃肠道疾病，它大大降低了患者的生活质量，并给社会带来经济负担。在以前的研究中，IBS患者直肠中表达降钙素的细胞密度与对照组没有差异。本研究旨在探讨肠易激综合征患者直肠5-羟色胺和选择性重摄取转运蛋白（SERT）的免疫反应强度。研究中包括50名IBS患者（41名女性和9名男性）。30例患者腹泻（IBS-D）和20例便秘（IBS-C）为主要症状。27例受试者作为对照（19例女性和8例男性）。直肠活检标本免疫染色使用抗生物素蛋白-生物素复合物法血清素和SERT。使用Olympus cell Sens成像软件通过计算机图像分析定量免疫反应性强度。在对照组、总IBS、IBS-D和IBS-C之间的多重比较中，5-羟色胺免疫反应强度无统计学差异。Dunn事后检验未发现四组之间存在任何统计学差异。在对照组、总IBS、IBS-D和IBS-C之间关于SERT免疫反应性强度的多重比较中存在显著的统计学差异。IBS-total、IBS-D和IBS-C的SERT免疫反应强度与对照组相比差异显著。得出的结论是，IBS患者的直肠SERT减少可能是导致这些患者腹泻和便秘发生的因素之一，并且越来越多的证据表明涉及SERT的遗传异常强调了SERT作用的重要性在IBS的病理生理学中。
Irritable bowel syndrome (IBS) is a common chronic gastrointestinal disorder, which considerably reduces the quality of life of patients and represents an economic burden to society. In previous studies, the density of serotonin-expressing cells in the rectum of IBS patients did not differ from that of control subjects. The present study was undertaken to investigate the immunoreactivity intensity of serotonin and serotonin-selective reuptake transporter (SERT) in the rectum of IBS patients. A cohort of 50 patients with IBS (41 females and 9 males) were included in the study. Thirty patients had diarrhoea (IBS-D) and 20 had constipation (IBS-C) as the predominant symptom. Twenty-seven subjects were included as controls (19 females and 8 males). Rectal biopsy specimens were immunostained using the avidin-biotin complex method for serotonin and SERT. The immunoreactivity intensity was quantified by computerised image analysis using Olympus cell Sens imaging software. There was no statistical difference of serotonin immunoreactivity intensity in multiple comparisons between controls, IBS-total, IBS-D and IBS-C. Dunn's post test did not reveal any statistical differences among the four groups. There was a significant statistical difference in multiple comparisons between controls, IBS-total, IBS-D and IBS-C regarding the SERT immunoreactivity intensity. SERT immunoreactivity intensity of IBS-total, IBS-D and IBS-C differed significantly from that of controls. It was concluded that the reduced rectal SERT in the IBS patients could be one of the factors contributing to the development of both diarrhoea and constipation in these patients, and that the increasing body of evidence of a genetic abnormality involving SERT underlines the importance of the role of SERT in the pathophysiology of IBS.