Automated flow cytometry for monitoring CHO cell cultures
Automated flow cytometry for monitoring CHO cell cultures
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DOI：
10.1016/j.ymeth.2012.03.001
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发表时间：
2012-03-01
期刊：
影响因子：
4.8
通讯作者：
Al-Rubeai, Mohamed
中科院分区：
文献类型：
--
作者：
Kuystermans, Darrin;Mohd, Avesh;Al-Rubeai, Mohamed
流式细胞术已被用于准确地监测指示生物反应器培养物的时空状态的细胞事件。过程分析技术（PAT）的引入已经导致使用实时或半实时监测系统的过程改进。将流式细胞仪集成到自动化方案中以改进过程监测，可以通过建立最佳工艺条件和更好的质量方案，使基于生物反应器的生物制药生产中的PAT受益。在此，我们提供了用于建立自动化流式细胞术系统的详细方案，该系统可用于研究和监测细胞生长、活力、细胞大小和细胞周期数据。描述了一种使用这种系统的方法，主要集中在CHO细胞培养上，尽管可以预见从自动流式细胞术收集的信息可以应用于各种细胞系，以解决PAT要求并获得对复杂生物系统的进一步理解。(C)2012 Elsevier Inc. All rights reserved.
Flow cytometry has been used to accurately monitor cell events that indicate the spatio-temporal state of a bioreactor culture. The introduction of process analytical technology (PAT) has led to process improvements using real-time or semi real-time monitoring systems. Integration of flow cytometry into an automated scheme for improved process monitoring can benefit PAT in bioreactor-based biopharmaceutical productions by establishing optimum process conditions and better quality protocols. Herein, we provide detailed protocols for establishing an automated flow cytometry system that can be used to investigate and monitor cell growth, viability, cell size, and cell cycle data. A method is described for the use of such a system primarily focused on CHO cell culture, although it is foreseen the information gathered from automated flow cytometry can be applied to a variety of cell lines to address both PAT requirements and gain further understanding of complex biological systems. (C) 2012 Elsevier Inc. All rights reserved.