Mutational analysis of endothelin receptor b1 (rose) during neural crest and pigment pattern development in the zebrafish Danio rerio
Mutational analysis of endothelin receptor b1 (rose) during neural crest and pigment pattern development in the zebrafish Danio rerio
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DOI：
10.1006/dbio.2000.9899
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发表时间：
2000-11-15
影响因子：
2.7
通讯作者：
Johnson, SL
中科院分区：
文献类型：
--
作者：
Parichy, DM;Mellgren, EM;Johnson, SL
鱼类的色素模式是研究胚后表型的遗传和细胞基础的一个易处理的系统。在斑马鱼Danio rerio中，神经嵴衍生的色素细胞在生命周期的不同阶段产生不同的色素模式。而早期幼虫表现出简单的黑素细胞和银虹膜细胞在黄色的黄色素的背景条纹，这种色素模式是在变态转化成成人，包括一系列黑暗的黑素细胞和虹膜细胞条纹，交替与虹膜细胞和黄色素的光条纹。虽然在D.虽然Rerio对早期幼虫和成虫色素模式的发育都有贡献，但对仅在一个或另一个生命周期阶段形成模式所必需的基因知之甚少。在这项研究中，我们确定了基因负责玫瑰突变表型在D。雷里奥。玫瑰突变体具有野生型早期幼虫的色素模式，但在色素模式变态期间不能发育正常数量的黑素细胞和虹膜细胞，并表现出这些细胞的破坏模式。我们发现，玫瑰对应于内皮素受体hi（ednrb1），一个直向同源物的nodote Ednrb基因，长期以来一直在研究他们的作用，在神经嵴和色素细胞的发展。此外，我们还证明了D.斑马鱼EDNRB 1在色素模式变态和胚胎发生过程中都表达，黑色素细胞、虹膜细胞和黄色素细胞谱系的细胞都表达该基因。这些分析表明Ednrb信号转导的作用，在发展中的鱼和硬骨鱼色素细胞前体的系统发育保守。由于Ednrb对所有神经嵴源性黑素细胞的发育都是必不可少的，而D. rerio ednrb 1仅为成年黑素细胞和虹膜细胞的一部分所需，这些分析也揭示了脊椎动物对Ednrb信号传导的细胞需求的差异，并为D.雷里奥。(C)北京大学出版社.
Pigment patterns of fishes are a tractable system for studying the genetic and cellular bases for postembryonic phenotypes. In the zebrafish Danio rerio, neural crest-derived pigment cells generate different pigment patterns during different phases of the life cycle. Whereas early larvae exhibit simple stripes of melanocytes and silver iridophores in a background of yellow xanthophores, this pigment pattern is transformed at metamorphosis into that of the adult, comprising a series of dark melanocyte and iridophore stripes, alternating with light stripes of iridophores and xanthophores. Although several genes have been identified in D. rerio that contribute to the development of both early larval and adult pigment patterns, comparatively little is known about genes that are essential for pattern formation during just one or the other life cycle phase. In this study, we identify the gene responsible for the rose mutant phenotype in D. rerio. rose mutants have wild-type early larval pigment patterns, but fail to develop normal numbers of melanocytes and iridophores during pigment pattern metamorphosis and exhibit a disrupted pattern of these cells. We show that rose corresponds to endothelin receptor hi (ednrb1), an orthologue of amniote Ednrb genes that have long been studied for their roles in neural crest and pigment cell development. Furthermore, we demonstrate that D. rerio ednrb1 is expressed both during pigment pattern metamorphosis and during embryogenesis, and cells of melanocyte, iridophore, and xanthophore lineages all express this gene. These analyses suggest a phylogenetic conservation of roles for Ednrb signaling in the development of amniote and teleost pigment cell precursors. As murine Ednrb is essential for the development of all neural crest derived melanocytes, and D. rerio ednrb1 is required only by a subset of adult melanocytes and iridophores, these analyses also reveal variation among vertebrates in the cellular requirements for Ednrb signaling, and suggest alternative models for the cellular and genetic bases of pigment pattern metamorphosis in D. rerio. (C) 2000 Academic Press.