DNA-passivated CdS nanocrystals: Luminescence, bioimaging, and toxicity profiles
DNA-passivated CdS nanocrystals: Luminescence, bioimaging, and toxicity profiles
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DOI：
10.1021/la7017727
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发表时间：
2007-12-18
期刊：
影响因子：
3.9
通讯作者：
Kelley, Shana O.
中科院分区：
文献类型：
--
作者：
Ma, Nan;Yang, Jun;Kelley, Shana O.
描述了一类发光半导体量子点，其在不使用大体积表面涂层的情况下表现出低细胞毒性。以单核苷酸或DNA寡核苷酸形式的核酸在CdS纳米晶体的水合成中用作配体系统。所产生的材料显示出约4 nm的直径和在500-700 nm范围内的发光。重要的是，DNA-CdS在高离子强度的缓冲液中稳定许多小时。当在HeLa细胞中测试毒性时，观察到细胞活力的最小降低，表明DNA-CdS纳米晶体在生物介质中高度稳定。测试纳米晶体到未固定的哺乳动物细胞中的摄取，并观察内化。报道的结果表明，使用DNA作为水溶性半导体纳米晶体的配体系统代表了生产新的生物成像剂的有价值的策略。
A class of luminescent semiconductor quantum dots is described that exhibit low cellular toxicity without the use of bulky surface coatings. Nucleic acids, either in the form of mononucleotides or DNA oligonucleotides, are used as a ligand system in the aqueous synthesis of CdS nanocrystals. The materials produced exhibit diameters on the order of 4 nm and luminescence in the range of 500-700 nm. Importantly, DNA-CdS is stable in buffers of high ionic strength for many hours. When tested for toxicity in HeLa cells, minimal decreases in cell viability were observed, indicating that the DNA-CdS nanocrystals are highly stable in biological media. Uptake of the nanocrystals into unfixed mammalian cells was tested, and internalization was observed. The results reported indicate that the use of DNA as a ligand system for water-soluble semiconductor nanocrystals represents a worthwhile strategy for the production of new biological imaging agents.