Optical Fiber Ring Cavity Sensor for Label-Free DNA Detection
Optical Fiber Ring Cavity Sensor for Label-Free DNA Detection
复制标题
DOI：
10.1109/jstqe.2011.2166110
复制
发表时间：
2012-05-01
影响因子：
4.9
通讯作者：
Pissadakis, Stavros
中科院分区：
文献类型：
--
作者：
Candiani, Alessandro;Sozzi, Michele;Pissadakis, Stavros
基于倾斜光纤布拉格光栅，开发了一种包层外敏化的无标记DNA生物传感器。该生物传感器用肽核酸（PNA）探针进行功能化，基于双倾斜光纤布拉格光栅，形成了一个改进的法布里 - 珀罗纤芯 - 包层闭环腔。在注入的导模与由倾斜光栅散射的光所产生的反射纤芯模和包层模之间形成干涉，导致在这两个光谱陷波内产生干涉条纹。当DNA与附着在光纤包层上的PNA探针结合时，在包层 - PNA界面处发生折射率变化，条纹可见度相应改变。报道了实时光谱测量结果，表明10 nM的DNA溶液会引起相应条纹可见度10%的调制。进行了循环测试以测量和检查传感器的重复性。
An outcladding sensitized label-free DNA biosensor is developed based on tilted fiber Bragg gratings. The biosensor, functionalized with peptide nucleic acid (PNA) probes, is based on a double tilted fiber Bragg grating that forms a modified Fabry-Perot core-cladding closed-loop cavity. Interference is set up between an injected guided mode and the reflected core mode and cladding modes created by the light scattered by the tilted gratings, leading to the generation of interference fringes within both these spectral notches. When the DNA binds to the PNA probes attached onto the fiber cladding, a refractive index change occurs at the cladding PNA interface and the fringe visibility changes accordingly. Real-time spectral measurement results are reported, showing that a 10-nM DNA solution induces a 10% modulation of the corresponding fringes visibility. Cycling tests are performed for measuring and checking the repeatability of the sensor.