Expansion of the genomics research on Atlantic salmon Salmo salar L. project (GRASP) microarray tools
Expansion of the genomics research on Atlantic salmon Salmo salar L. project (GRASP) microarray tools
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大西洋鲑鱼 Salmo salar L. 项目 (GRASP) 微阵列工具的基因组学研究扩展
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
B. Koop
中科院分区：
文献类型：
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作者：
K. R. VonSchalburg;G. Cooper;Jong S Leong;A. Robb;R. Lieph;M. Rise;W. Davidson;B. Koop
鲑鱼是研究最广泛的鱼类，在过去的几年里，基因组学技术已经开始为这种丰富的生物学做出贡献。第一个鲑鱼微阵列出现在2004年，从那时起，几十项研究已经证明了基因组方法的实用性。大西洋鲑基因组学研究项目16 k阵列的广泛应用和基因组资源的极大扩展，使得实验性的5 k oligo（70-mer）阵列和32 k cDNA阵列在不久的将来得以发展。在本文中，作者检查了过去阵列开发中使用的一些程序，并根据新的基因组数据对其进行了重新检查。研究了新的5 k阵列的一些初步对照实验，其基于与polyA尾的距离、错配的影响和跨物种杂交特异性来检查寡核苷酸设计。在新的32 k cDNA阵列的开发中也确定了有益的方法。
Salmonids are the most widely studied group of fish, and in the last few years, genomics technologies have begun to contribute to this rich biology. The first salmonid microarrays appeared in 2004 and since then several dozen studies have demonstrated the utility of genomic approaches. The widespread use of the genomics research on Atlantic salmon project 16 k array and greatly expanded genome resources have led to the development of an experimental 5 k oligo (70-mer) array and a 32 k cDNA microarray in the near future. In this paper, the authors examined some of the procedures used in the development of past arrays and reexamined them in light of new genomic data available. Some preliminary control experiments of the new 5 k array were investigated that examine oligo designs based on distance from the polyA tail, the effects of mismatches and cross-species hybridization specificity. Beneficial approaches are also identified in the development of the new 32 k cDNA array.
使用虹鳟鱼寡核苷酸微阵列确定黄曲霉毒素 B1 诱导的肝细胞癌与邻近肝脏的转录模式。
DOI：
10.1093/toxsci/kfi309
发表时间：
2005
期刊：
Toxicological sciences : an official journal of the Society of Toxicology
影响因子：
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作者：
Tilton,SusanC;Gerwick,LenaG;Hendricks,JerryD;Rosato,CapriceS;Corley-Smith,Graham;Givan,ScottA;Bailey,GeorgeS;Bayne,ChristopherJ;Williams,DavidE
通讯作者：
Williams,DavidE