Application of the cell sheet technique in tissue engineering
Application of the cell sheet technique in tissue engineering
复制标题
细胞片层技术在组织工程中的应用
DOI：
10.3892/br.2015.522
复制
发表时间：
2015-11-01
影响因子：
2.3
通讯作者：
Yan, Weiqi
中科院分区：
文献类型：
--
作者：
Chen, Guangnan;Qi, Yiying;Yan, Weiqi
组织工程技术的发展和应用在再生医学领域显示出巨大的潜力。然而，传统的组织工程方法（细胞悬液、支架和/或生长因子）的局限性限制了其在某些领域的应用。新的细胞片技术可以克服这些缺点。培养的细胞可以作为完整的片收获，而不使用蛋白水解酶，如胰蛋白酶或分散酶，这可能导致细胞损伤和分化表型的损失。细胞片层是一个完整的细胞层，含有细胞外基质、离子通道、生长因子受体、连接蛋白等重要的细胞表面蛋白。骨髓间充质干细胞具有多向分化潜能，是组织工程的理想种子细胞。MSC片材技术可能在再生医学和组织工程领域具有潜力。此外，已提出诱导多能干细胞和胚胎干细胞衍生的细胞片用于组织再生。目前，细胞片在组织重建中的应用包括：直接植入受体部位，细胞片叠加构建三维结构用于植入，或细胞片与支架结合。本文综述了细胞片层技术，特别是干细胞片层技术在组织工程中的应用进展。
The development and application of the tissue engineering technique has shown a significant potential in regenerative medicine. However, the limitations of conventional tissue engineering methods (cell suspensions, scaffolds and/or growth factors) restrict its application in certain fields. The novel cell sheet technique can overcome such disadvantages. Cultured cells can be harvested as intact sheets without the use of proteolytic enzymes, such as trypsin or dispase, which can result in cell damage and loss of differentiated phenotypes. The cell sheet is a complete layer, which contains extracellular matrix, ion channel, growth factor receptors, nexin and other important cell surface proteins. Mesenchymal stem cells (MSCs), which have the potential for multiple differentiation, are promising candidate seed cells for tissue engineering. The MSC sheet technique may have potential in the fields of regenerative medicine and tissue engineering in general. Additionally, induced pluripotent stem cell and embryonic stem cell-derived cell sheets have been proposed for tissue regeneration. Currently, the application of cell sheet for tissue reconstruction includes: Direct recipient sites implantation, superposition of cell sheets to construct three-dimensional structure for implantation, or cell sheet combined with scaffolds. The present review discusses the progress in cell sheet techniques, particularly stem cell sheet techniques, in tissue engineering.