Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
Identification and analysis of the β-catenin1 gene in half-smooth tongue sole (Cynoglossus semilaevis).
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半滑舌鳎β-catenin1基因的鉴定与分析
DOI：
10.1371/journal.pone.0176122
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Zhu Y;Hu Q;Xu W;Li H;Guo H;Meng L;Wei M;Lu S;Shao C;Wang N;Yang G;Chen S
β-catenin是典型Wnt通路中的关键信号分子，在细胞粘附、胚胎发生和性别决定中发挥作用。然而，人们对它在硬骨鱼中的作用知之甚少。我们从半光滑舌底（Cynoglossus semilaevis）中克隆并鉴定了β-catenin1全长基因，命名为CS-β-catenin1。CS-β-catenin1 cDNA由2346个核苷酸组成，编码一个含有782个氨基酸的蛋白质。尽管CS-β-catenin1在两性性腺中均有转录，但其在卵巢中的表达水平明显高于睾丸。CS-β-catenin1 mRNA水平在160日龄显著上调，并持续升高至2岁。原位杂交显示CS-β- catenin1mrna主要定位于卵母细胞，特别是在I、II和III期卵母细胞中。在卵巢注射槲皮素抑制CS-β-catenin1表达后，CS- figla和CS-foxl2 mRNA水平显著下调，CS-dmrt1水平上调，提示CS-β-catenin1可能是CS- figla的上游基因，参与卵巢发育，即卵泡发生。
β-catenin is a key signalling molecule in the canonical Wnt pathway, which plays a role in cell adhesion, embryogenesis and sex determination. However, little is known about its function in teleosts. We cloned and characterized the full-length β-catenin1 gene from half-smooth tongue sole (Cynoglossus semilaevis), which was designated CS-β-catenin1. The CS-β-catenin1 cDNA consists of 2,346 nucleotides and encodes a protein with 782 amino acids. Although CS-β-catenin1 was transcribed in the gonads of both sexes, the level was significantly higher in ovaries compared to testes. Furthermore, the mRNA level of CS-β-catenin1 was significantly upregulated at 160 days and constantly increased until 2 years of age. In situ hybridization revealed that CS-β-catenin1 mRNA was mainly localized in oocyte cells, especially in stage I, II and III oocytes. When CS-β-catenin1 expression was inhibited by injection of quercetin in the ovaries, levels of CS-Figla and CS-foxl2 mRNA were significantly down-regulated, and CS-dmrt1 was up-regulated, which suggested that CS-β-catenin1 is a potential upstream gene of CS-Figla and is involved in the development of the ovaries, i.e., folliculogenesis.