Origin, expression and possible functions of the two alternatively spliced forms of the mouse Nedd2 mRNA
Origin, expression and possible functions of the two alternatively spliced forms of the mouse Nedd2 mRNA
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DOI：
10.1038/sj.cdd.4400251
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发表时间：
1997-07-01
影响因子：
12.4
通讯作者：
Noda, M
中科院分区：
文献类型：
--
作者：
Kumar, S;Kinoshita, M;Noda, M
Nedd2基因是ICE/ced-3家族的一员，通过差异剪接转录两种mRNA。其中一种编码一种较短的被截断的蛋白质，称为Nedd2s/ICH-1s。Nedd2/ICH-1诱导细胞凋亡，而ICH-1被认为是细胞死亡的负调控因子。在这次通信中，我们分析了Nedd2s/ICH-1s的各个方面。小鼠Nedd2的编码区由10个外显子组成，第7个外显子的选择性剪接导致两个转录本的产生。虽然Nedd2s的表达在NIH-3T3细胞中对血清停药诱导的细胞凋亡具有部分抗性，但它不能阻止Nedd2过表达诱导的细胞死亡，并且在因子剥夺条件下，在另外两种细胞系（N18和FDC-P1）中不能抑制细胞凋亡。我们的研究结果表明，Nedd2s/ICH-1s不是细胞死亡的一般调节剂。
The Nedd2 gene, a member of the ICE/ced-3 family, transcribes two mRNA species by differential splicing. One of these encodes a shorter truncated version of the protein termed Nedd2s/ICH-1s. While Nedd2/ICH-1 induces apoptosis, ICH-1s was proposed to be a negative regulator of cell death. In this communication we have analyzed various aspects of the Nedd2s/ICH-1s. The coding region of mouse Nedd2 consists of 10 exons and alternative splicing of the 7th exon results in the generation of two transcripts. Although Nedd2s expression conferred partial resistance to serum withdrawal-induced apoptosis in NIH-3T3 cells, it failed to prevent cell death induced by the overexpression of Nedd2 and was unable to suppress apoptosis in two other cell lines (N18 and FDC-P1) under factor deprived conditions. Our results indicate that Nedd2s/ICH-1s is not a general modulator of cell death.