Leukotriene C4 inhibits ATP‐dependent transport of glutathione S‐conjugate across rat heart sarcolemma Implication for leukotriene C4 translocation mediated by glutathione S‐conjugate carrier
Leukotriene C4 inhibits ATP‐dependent transport of glutathione S‐conjugate across rat heart sarcolemma Implication for leukotriene C4 translocation mediated by glutathione S‐conjugate carrier
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白三烯 C4 抑制谷胱甘肽 S 结合物通过大鼠心脏肌膜的 ATP 依赖性转运 谷胱甘肽 S 结合物载体介导的白三烯 C4 易位的意义
DOI：
10.1016/0014-5793(89)80278-9
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发表时间：
1989
期刊：
影响因子：
3.5
通讯作者：
T. Ishikawa
中科院分区：
文献类型：
--
作者：
T. Ishikawa
从大鼠心脏制备的肌膜囊泡表现出S-（2，4-二硝基苯基）谷胱甘肽（DNP-SG）的ATP依赖性摄取，其符合米氏动力学，DNP-SG的表观Km为21 μM，Vmax为0.27 nmol · 10 min−1· mg protein−1。几种模型谷胱甘肽S-结合物抑制DNP-SG摄取，但即使在较低浓度下，白三烯C4也能更显著地抑制摄取（竞争性抑制，Ki= 1.5 μM）。然而，缺乏γ-谷氨酰部分的白三烯D4和E4效果较差。结果表明，ATP依赖性转运系统对白三烯C4具有高亲和力，可能是该化合物转运的原因。
Sarcolemmal vesicles prepared from rat heart exhibited ATP‐dependent uptake ofS‐(2,4‐dinitrophenyl)glutathione (DNP‐SG), which obeyed Michaelis‐Menten kinetics with an apparentKmof 21 μM for DNP‐SG and aVmaxof 0.27 nmol · 10 min−1· mg protein−1. Several model glutathioneS‐conjugates inhibited DNP‐SG uptake, but leukotriene C4inhibited uptake much more significantly even at lower concentrations (competitive inhibition,Ki= 1.5 μM). However, leukotrienes D4and E4, which lack the γ‐glutamyl moiety, were less effective. The results suggest that the ATP‐dependent transport system has a high affinity for leukotriene C4, and may be responsible for the translocation of this compound.