Chondrogenesis enhanced by overexpression of sox9 gene in mouse bone marrow-derived mesenchymal stem cells
Chondrogenesis enhanced by overexpression of sox9 gene in mouse bone marrow-derived mesenchymal stem cells
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DOI：
10.1016/s0006-291x(02)03026-7
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发表时间：
2003-02-07
影响因子：
3.1
通讯作者：
Ishiguro, N
中科院分区：
文献类型：
--
作者：
Tsuchiya, H;Kitoh, H;Ishiguro, N
我们研究了细胞介导的sox 9基因治疗作为一种新的软骨再生治疗方案的软骨发生。通过脂质体转染法将含有全长小鼠sox 9 cDNA的pIRES 2-EGFP载体转染入骨髓间充质干细胞（MSCs）中，并评价这些细胞的软骨分化。体外高密度微团培养结果显示，转染sox 9基因的骨髓间充质干细胞聚集成富含基质的微团，且表达EGFP的骨髓间充质干细胞被Alcian蓝和II型胶原染色阳性。接下来，将sox 9转染的MSC加载到扩散室中并移植到无胸腺小鼠中以分析体内软骨形成。移植4周后，在腔室中可见直径约2 mm的大块组织形成。组织学检查表明，阿新蓝和II型胶原阳性染色的细胞外基质的肿块，而X型胶原不染色。这些结果表明，细胞介导的sox 9基因治疗透明软骨损伤可能是一种新的策略。(C)2003 Elsevier Science（美国）。All rights reserved.
We investigated chondrogenesis of cell-mediated sox9 gene therapy as a new treatment regimen for cartilage regeneration. pIRES2-EGFP vector containing a full-length mouse sox9 cDNA was transfected into bone marrow-derived mesenchymal stem cells (MSCs) by lipofection and chondrogenic differentiation of these cells was evaluated. In vitro high density micromass culture of these sox9 transfected MSCs demonstrated that a matrix-rich micromass aggregate with EGFP expressing MSCs was positively stained by Alcian blue and type II collagen. Next, sox9 transfected MSCs were loaded into the diffusion chamber and transplanted into athymic mice to analyze in vivo chondrogenesis. A massive tissue formation in about 2 mm diameter was visible in the chamber after 4 weeks transplantation. Histological examinations demonstrated that both Alcian blue and type II collagen were positively stained in the extracellular matrix of the mass while type X collagen was not stained. These results indicated that cell-mediated sox9 gene therapy could be a novel strategy for hyaline cartilage damage. (C) 2003 Elsevier Science (USA). All rights reserved.