Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum.
Lipidomic Analysis of Extracellular Vesicles Isolated from Human Plasma and Serum.
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从人血浆和血清中分离的细胞外囊泡的脂质组学分析。
DOI：
10.1007/978-1-0716-2341-1_12
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发表时间：
2022
影响因子：
--
通讯作者：
Saito K. and Saito Y
中科院分区：
文献类型：
--
作者：
Sun Y.;Saito K. and Saito Y
脂质组学是一种全面研究生物样本，如血浆、血清、尿液和组织样本中的脂类分布的组学方法。此外，脂肪组学分析有助于识别新的脂质生物标记物，特别是对人类各种代谢性和恶性疾病。细胞外小泡(EVS)是脂双层包裹的纳米颗粒，从各种细胞分泌到细胞外空间。特别是，血流中循环的EV已经引起了相当大的研究兴趣，因为它们被认为是分泌EV的细胞的指纹，是一种有希望的较小侵入性生物标记物筛选的来源。在这里，我们描述了循环中EV的脂组分析的整个工作流程，包括从人体血浆和血清中提纯EV的方法，基于高分辨率质谱仪的脂类测量的高效液相色谱，以及EV脂类的数据分析。使用这种方法工作流程，可以检测到260多个属于甘油磷脂和鞘磷脂基团的脂分子。
Lipidomics is an omics approach to comprehensively study lipid profiles in biological samples, such as plasma, serum, urine, and tissue specimens. Moreover, lipidomic analyses are useful for identifying novel lipid biomarkers, especially for various metabolic and malignant diseases in humans. Extracellular vesicles (EVs) are lipid bilayer-encapsulated nanoparticles secreted from various cells into the extracellular space. In particular, circulating EVs in the blood stream have attracted considerable research interest as they are considered the fingerprint of the cells from which they are secreted and are a promising source for less-invasive biomarker screening. Here, we describe the entire workflow for the lipidomic analysis of circulating EVs, including the methods for their purification from human plasma and serum, liquid chromatography coupled with high-resolution mass spectrometry-based lipid measurement, and data analyses for profiling EV lipids. Using this methodological workflow, over 260 lipid molecules belonging to the glycerophospholipid and sphingolipid groups can be detected.