[Studies on biological effects of kappa-selenocarrageenan on human breast cancer cell line BCaP-37].
[Studies on biological effects of kappa-selenocarrageenan on human breast cancer cell line BCaP-37].
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发表时间：
1997-03
影响因子：
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通讯作者：
G. He;C. Cheng;R. Lu
中科院分区：
文献类型：
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作者：
G. He;C. Cheng;R. Lu
应用细胞培养技术、图像细胞仪（ICM）和北方印迹法（Northern blot）检测了硒化卡拉胶对中国人乳腺癌细胞株BCaP-37增殖、细胞周期、DNA总量、细胞核面积、表皮生长因子受体（EGFR）和癌基因C-erbB 2 mRNA表达的影响，以探讨硒化卡拉胶的抗肿瘤机制。结果表明，3.0 ~ 120 mg/L的硒化卡拉胶对BCaP-37细胞的增殖有明显的抑制作用，且呈时间和剂量依赖性。15和60 mg/L硒角叉菜胶处理4d后，ICM细胞核面积显著低于对照组（P < 0.01）。60 mg/L硒化卡拉胶可显著抑制EGFR水平和C-erbB 2 mRNA表达。提示硒化卡拉胶可通过调节EGFR水平和C-erbB 2 mRNA表达抑制乳腺癌细胞增殖。
Proliferation, cell cycle, total amount of DNA, area of cell nucleus, as well as epidermal growth factor receptors (EGFR) and expression of oncogene C-erbB2 mRNA of Chinese breast cancer cell line (BCaP-37) after being treated with kappa-selenocarrageenan were determined by cell culture technique, image cytometry (ICM) and northern blot to explore its anti-tumor mechanism. Results revealed 3.0-120 mg/L selenocarrageenan could inhibit proliferation of BCaP-37, with a response of time and dose dependence. The areas of nuclei were significantly lower with ICM in cells treated with 15 or 60 mg/L selenocarrageenan for four days than those in controls (P < 0.01). Levels of EGFR and expression of C-erbB2 mRNA were significantly inhibited in cells treated with 60 mg/L selenocarrageenan. It suggests that selenocarrageenan can inhibit proliferation of breast cancer cells through regulation of the levels of EGFR and expression of C-erbB2 mRNA.