Crystallization of a complex between human CDK6 and a virus-encoded cyclin is critically dependent on the addition of small charged organic molecules.
Crystallization of a complex between human CDK6 and a virus-encoded cyclin is critically dependent on the addition of small charged organic molecules.
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人类 CDK6 和病毒编码的细胞周期蛋白之间的复合物的结晶很大程度上取决于带电有机小分子的添加。
DOI：
10.1107/s0907444901009283
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Kim,SH
中科院分区：
文献类型：
--
作者：
Schulze-Gahmen,U;Kim,SH
人CDK6在控制进入真核细胞周期中起重要作用。将人CDK6与来自松鼠猴疱疹病毒的病毒细胞周期蛋白的活化复合物纯化至均一，并使用聚乙二醇3350作为沉淀剂进行结晶。结晶是严格依赖于一个狭窄的范围内的醋酸钙浓度和存在的磺基甜菜碱201作为添加剂。晶体属于六方晶系空间群P6122或P6522，晶胞参数a = B = 70.14，c = 448.77 μ m，γ = 120°，X射线衍射分辨率至少为3.1 μ m。
Human CDK6 plays an important role in controlling entry into the eukaryotic cell cycle. An activated complex of human CDK6 with a viral cyclin from herpesvirus saimiri was purified to homogeneity and crystallized using polyethylene glycol 3350 as precipitant. Crystallization was critically dependent on a narrow range of calcium acetate concentration and the presence of sulfo-betaine 201 as additive. Crystals belong to the hexagonal space group P6122 or P6522, with unit-cell parameters a = b = 70.14, c = 448.77 Å, γ = 120°, and diffract X-rays to at least 3.1 Å resolution.