Human Cdc45 is a proliferation-associated antigen
Human Cdc45 is a proliferation-associated antigen
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DOI：
10.1111/j.1742-4658.2007.05900.x
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发表时间：
2007-07-01
期刊：
影响因子：
5.4
通讯作者：
Grosse, F.
中科院分区：
文献类型：
--
作者：
Pollok, S.;Bauerschmidt, C.;Grosse, F.
细胞分裂周期蛋白45 （Cdc45）在DNA复制中起关键作用，确保染色体DNA在每个细胞周期中只复制一次。我们分析了人Cdc45在增殖和非增殖细胞中的表达。我们的研究结果表明，Cdc45蛋白不存在于长期静止、终末分化和衰老的人类细胞中，尽管它存在于增殖细胞的整个细胞周期中。此外，在人类细胞中，Cdc45的丰度远低于微染色体维持（Mcm）蛋白，这支持了Cdc45的起源结合是复制起始速率限制的概念。我们还表明，与原代人类细胞相比，人类癌症来源细胞中的Cdc45蛋白水平始终较高。因此，肿瘤组织优先使用cdc45特异性抗体染色。因此，Cdc45的表达与增殖细胞群密切相关，Cdc45似乎是癌细胞生物学中一种有希望的新型增殖标志物。
Cell division cycle protein 45 (Cdc45) plays a critical role in DNA replication to ensure that chromosomal DNA is replicated only once per cell cycle. We analysed the expression of human Cdc45 in proliferating and nonproliferating cells. Our findings show that Cdc45 protein is absent from long-term quiescent, terminally differentiated and senescent human cells, although it is present throughout the cell cycle of proliferating cells. Moreover, Cdc45 is much less abundant than the minichromosome maintenance (Mcm) proteins in human cells, supporting the concept that origin binding of Cdc45 is rate limiting for replication initiation. We also show that the Cdc45 protein level is consistently higher in human cancer-derived cells compared with primary human cells. Consequently, tumour tissue is preferentially stained using Cdc45-specific antibodies. Thus, Cdc45 expression is tightly associated with proliferating cell populations and Cdc45 seems to be a promising candidate for a novel proliferation marker in cancer cell biology.