MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins
MRPS27 is a pentatricopeptide repeat domain protein required for the translation of mitochondrially encoded proteins
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DOI：
10.1016/j.febslet.2012.07.043
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发表时间：
2012-10-19
期刊：
影响因子：
3.5
通讯作者：
Filipovska, Aleksandra
中科院分区：
文献类型：
--
作者：
Davies, Stefan M. K.;Sanchez, Maria I. G. Lopez;Filipovska, Aleksandra
哺乳动物五肽重复结构域（PPR）蛋白参与线粒体RNA代谢和翻译的调节，并且是线粒体功能所需的。我们研究了一种未表征的PPR蛋白，即小亚基27（MRPS27）的额外线粒体核糖体蛋白，并表明它与12S rRNA和tRNA（Glu）相关，但它不影响它们的丰度。我们发现MRPS27不是线粒体RNA加工或核糖体小亚基稳定性所必需的。然而，MRPS27是线粒体蛋白质合成所必需的，其敲低导致呼吸复合物和细胞色素c氧化酶活性的丰度降低。
Mammalian pentatricopeptide repeat domain (PPR) proteins are involved in regulation of mitochondrial RNA metabolism and translation and are required for mitochondrial function. We investigated an uncharacterised PPR protein, the supernumerary mitochondrial ribosomal protein of the small subunit 27 (MRPS27), and show that it associates with the 12S rRNA and tRNA(Glu), however it does not affect their abundance. We found that MRPS27 is not required for mitochondrial RNA processing or the stability of the small ribosomal subunit. However, MRPS27 is required for mitochondrial protein synthesis and its knockdown causes decreased abundance in respiratory complexes and cytochrome c oxidase activity.