Spontaneous and induced synthesis of cytokines by peripheral blood monocytes in patients with endometriosis
Spontaneous and induced synthesis of cytokines by peripheral blood monocytes in patients with endometriosis
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DOI：
10.1016/s0015-0282(16)58325-3
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发表时间：
1996-06-01
影响因子：
6.7
通讯作者：
Dmowski, WP
中科院分区：
文献类型：
--
作者：
Braun, DP;Gebel, H;Dmowski, WP
目的：探讨子宫内膜异位症患者外周血单核细胞分泌肿瘤坏死因子-α的能力设计：在存在和不存在脂多糖的情况下培养外周血单核细胞24小时，然后通过酶免疫测定法评估上清液中的细胞因子。子宫内膜异位症研究和治疗研究所和大学研究实验室。患者和参与者：生育对照组，n = 10;子宫内膜异位症妇女，n = 20。干预措施：无。结果：与生育对照组相比，子宫内膜异位症妇女PBM的TNF-α、IL-6和IL-8的基础合成以及脂多糖刺激的TNF-α合成显著增加。结论：子宫内膜异位症患者PBM基础和刺激性IL-10的合成和分泌增加，与子宫内膜异位症患者PBM基础和刺激性IL-10的合成和分泌增加有关。发现受影响的每种细胞因子都有能力在疾病的病理学或发病机制中发挥作用。
Objective: To investigate the capacity of peripheral blood monocytes (PBM) from women with endometriosis to secrete tumor necrosis factor-alpha (TNF-alpha), interleukin (IL) IL-6, IL-8, and IL-10.Design: Peripheral blood monocytes were cultured in the presence and absence of lipopolysaccharide for 24 hours before assessment of cytokines in supernatants by enzyme immunoassay.Setting: Institute for the Study and Treatment of Endometriosis and university-based research laboratories.Patients and Participants: Fertile controls, n = 10; women with endometriosis, n = 20.Interventions: None.Results: Basal synthesis of TNF-alpha, IL-6, and IL-8 and the Lipopolysaccharide-stimulated synthesis of TNF-alpha by PBM from women with endometriosis was significantly greater in comparison to that of fertile controls. Endometriosis had no effect on the basal or stimulated synthesis of IL-10.Conclusions: These data show that endometriosis is associated with increased basal and stimulated synthesis and secretion of several different cytokines by PBM. Each of the cytokines found to be affected has the capacity to play a role in the symptomatology or pathogenesis of the disease.