Inducing precocious anaphase in cultured mammalian cells
Inducing precocious anaphase in cultured mammalian cells
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DOI：
10.1002/cm.10032
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发表时间：
2002-06-01
影响因子：
--
通讯作者：
Fang, GW
中科院分区：
文献类型：
--
作者：
Canman, JC;Salmon, ED;Fang, GW
纺锤体检查点可以防止纺锤体损伤或染色体不正确排列导致的后期发生，这对后期和细胞质分裂中蛋白质功能的实验分析提出了一个问题。这是因为在后期开始前许多蛋白质的功能破坏可以激活这个检查点，阻止后期和随后的细胞周期事件。本文比较了在哺乳动物组织培养细胞中覆盖梭形检查点的新方法和旧方法。(C) 2002 Wiley-Liss。公司。
The spindle checkpoint, which prevents anaphase onset upon spindle damage or incorrect chromosome alignment, presents a problem for experimental analysis of protein function in anaphase and cytokinesis. This is because the functional disruption of many proteins before anaphase onset can activate this checkpoint, preventing anaphase and subsequent cell cycle events. This paper compares new and old methods of overriding the spindle checkpoint in prometaphase mammalian tissue culture cells. (C) 2002 Wiley-Liss. Inc.