Ghrelin is suppressed by glucagon and does not mediate glucagon-related growth hormone release.
Ghrelin is suppressed by glucagon and does not mediate glucagon-related growth hormone release.
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生长素释放肽被胰高血糖素抑制，并且不介导胰高血糖素相关的生长激素的释放。
DOI：
10.1159/000084569
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Chanoine,Jean-Pierre
中科院分区：
文献类型：
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作者：
Hirsh,Denton;Heinrichs,Claudine;Leenders,Bert;Wong,AlfredCK;Cummings,DavidE;Chanoine,Jean-Pierre
背景胰高血糖素刺激是儿科常规的评估生长激素（GH）充足性的激发试验。Ghrelin还显著刺激GH分泌。由于胰高血糖素刺激的促生长素基因的启动子在体外以及胃促生长素分泌灌注大鼠胃，我们试图确定是否胃促生长素介导胰高血糖素诱导的GH secretory.MethodsWe比较胃促生长素，生长激素，胰岛素和葡萄糖反应后，0.03毫克/公斤静脉注射（iv;最大。1 mg）和0.1 mg/kg肌内（im; max. 2 mg）胰高血糖素治疗两组GH-充足儿童（n= 10-11/组）。我们还测量了胃饥饿素之前和6分钟后，静脉注射1毫克胰高血糖素在21名成人subjects.ResultsIn儿童，胰高血糖素引起的胃饥饿素减少26%，葡萄糖浓度增加72%，是独立的剂量或给药途径的胰高血糖素。相比之下，与0.03 mg/kg胰高血糖素iv给药相比，0.1 mg/kg im给药后的胰岛素反应高2-3倍。生长激素释放肽的最大降低与葡萄糖的增加之间存在显著相关性（p= 0.03），但与胰岛素无关。在控制胰高血糖素剂量后，生长激素释放肽和GH曲线下面积之间存在显著相关性（p= 0.03），但葡萄糖的最大增加量无关。在正常成人中，胰高血糖素给药引起了7%的减少，6 min后的生长激素释放肽浓度（p= 0.0002）。ConclusionGhrelin不发挥因果作用的GH响应药理胰高血糖素给药，抑制生长激素释放肽水平开始注射后几分钟。
BackgroundGlucagon stimulation is routinely used as a provocative test to assess growth hormone (GH) sufficiency in pediatrics. Ghrelin also markedly stimulates GH secretion. Because glucagon stimulates the promoter of the ghrelin gene in vitro as well as ghrelin secretion by the perfused rat stomach, we sought todetermine whether ghrelin mediates glucagon-induced GH secretion.MethodsWe compared ghrelin, GH, insulin and glucose responses following administration of 0.03 mg/kg intravenously (iv; max. 1 mg) and 0.1 mg/kg intramuscularly (im; max. 2 mg) of glucagon in two groups (n= 10–11/group) of GH-sufficient children. We also measured ghrelin before and 6 min after iv administration of 1 mg glucagon in 21 adult subjects.ResultsIn children, glucagon caused a 26% decrease in ghrelin and a 72% increase in glucose concentrations that were independent of the dose or administration route of glucagon. In contrast, the insulin response was 2–3 times higher following administration of 0.1 mg/kg im compared to 0.03 mg/kg of glucagon iv. There was a significant correlation between the maximum decrease in ghrelin and increases in glucose (p= 0.03) but not in insulin. There was a significant correlation between ghrelin and GH area under the curve after controlling for the dose of glucagon (p= 0.03) but not for the maximum increase in glucose. In normal adults, glucagon administration caused a 7% decrease in ghrelin concentrations after 6 min (p= 0.0002).ConclusionGhrelin does not play a causal role in the GH response to pharmacological glucagon administration, which suppresses ghrelin levels starting a few minutes after injection.