Transcriptome and biomineralization responses of the pearl oyster Pinctada fucata to elevated CO2 and temperature.
Transcriptome and biomineralization responses of the pearl oyster Pinctada fucata to elevated CO2 and temperature.
复制标题
珍珠贝 Pinctada fucata 对二氧化碳和温度升高的转录组和生物矿化反应
DOI：
10.1038/srep18943
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发表时间：
2016-01-06
影响因子：
4.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Li S;Liu C;Huang J;Liu Y;Zhang S;Zheng G;Xie L;Zhang R
海洋酸化和全球变暖已被证明显著影响海洋钙化剂的生理性能；然而，潜在的机制仍然知之甚少。在这项研究中，研究了fucatada在升高的CO2（pH 7.8和pH 7.5）和温度（25°C和31°C）下的转录组和生物矿化反应。co2和温度的升高引起基因表达、碱性磷酸酶活性、净钙化率和相对钙含量的显著变化，而壳的超微结构没有变化。“离子和酸碱调节”相关基因和“氨基酸代谢”途径对CO2升高（pH值7.8）有响应。此外，“抗氧化”相关基因和“toll样受体信号传导”、“花生四烯酸代谢”、“溶酶体”和“其他聚糖降解”途径对高温（25°C和31°C）表现出响应，表明p。利用抗氧化和溶酶体策略减轻温度应激的影响。这些反应是消耗能量的过程，可导致生物矿化能力下降。因此，这项研究对于了解珍珠牡蛎对环境变化的响应机制和预测全球气候变化对珍珠养殖的影响具有重要意义。
Ocean acidification and global warming have been shown to significantly affect the physiological performances of marine calcifiers; however, the underlying mechanisms remain poorly understood. In this study, the transcriptome and biomineralization responses ofPinctada fucatato elevated CO2(pH 7.8 and pH 7.5) and temperature (25 °C and 31 °C) are investigated. Increases in CO2and temperature induced significant changes in gene expression, alkaline phosphatase activity, net calcification rates and relative calcium content, whereas no changes are observed in the shell ultrastructure. “Ion and acid-base regulation” related genes and “amino acid metabolism” pathway respond to the elevated CO2(pH 7.8), suggesting thatP. fucataimplements a compensatory acid-base mechanism to mitigate the effects of low pH. Additionally, “anti-oxidation”-related genes and “Toll-like receptor signaling”, “arachidonic acid metabolism”, “lysosome” and “other glycan degradation” pathways exhibited responses to elevated temperature (25 °C and 31 °C), suggesting thatP. fucatautilizes anti-oxidative and lysosome strategies to alleviate the effects of temperature stress. These responses are energy-consuming processes, which can lead to a decrease in biomineralization capacity. This study therefore is important for understanding the mechanisms by which pearl oysters respond to changing environments and predicting the effects of global climate change on pearl aquaculture.