K+ self-exchange by the Na+ pump: regulation by P(i) and metabolic perturbations.
K+ self-exchange by the Na+ pump: regulation by P(i) and metabolic perturbations.
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Na 泵的 K 自交换：P(i) 和代谢扰动的调节。
DOI：
10.1152/ajpcell.1995.269.1.c170
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Stokes,JB
中科院分区：
文献类型：
--
作者：
Nonaka,T;Warden,DH;Matsushita,K;Stokes,JB
我们以前已经证明，在兔皮质集合管的基底外侧膜上的Na（+）-K+泵可以在K+/K+交换模式中起作用。增加红细胞中的细胞内磷酸盐会抑制Na+泵并增加K+/K+交换。我们发现，旨在增加集合管细胞内磷酸盐的演习引起K+/K+交换的增加。使细胞经受代谢性损伤（氰化物）增加了通过泵的K+/K+交换，如通过其哇巴因敏感性和缺乏产电或导电特性所判断的。结果表明，通过Na（+）-K+泵的K+/K+交换速率可以通过在生理学或病理学上可实现的范围内的细胞内磷酸盐的变化来改变。结果还表明，在代谢应激过程中抑制矢量Na+运输的机制。
We have previously demonstrated that the Na(+)-K+ pump on the basolateral membrane of the rabbit cortical collecting duct can function in the K+/K+ exchange mode. Increasing intracellular phosphate in red blood cells inhibits the Na+ pump and increases K+/K+ exchange. We found that maneuvers designed to increase intracellular phosphate in collecting duct cells caused an increase in K+/K+ exchange. Subjecting the cells to a metabolic insult (cyanide) increased K+/K+ exchange by the pump as judged by its ouabain sensitivity and lack of electrogenic or conductive characteristics. The results demonstrate that the rate of K+/K+ exchange by the Na(+)-K+ pump can be altered by changes in intracellular phosphate over a range that is physiologically or pathologically achievable. The results also suggest a mechanism for inhibition of vectorial Na+ transport during metabolic stress.