Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barre Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds
Differential Distribution of the wlaN and cgtB Genes, Associated with Guillain-Barre Syndrome, in Campylobacter jejuni Isolates from Humans, Broiler Chickens, and Wild Birds
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DOI：
10.3390/microorganisms8030325
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发表时间：
2020-03-01
期刊：
影响因子：
4.5
通讯作者：
Madrid, Cristina
中科院分区：
文献类型：
--
作者：
Guirado, Pedro;Paytubi, Sonia;Madrid, Cristina
空肠弯曲杆菌引起弯曲杆菌病，这是一种在世界范围内发病率很高的细菌性胃肠炎。此外，C.空肠感染可引发称为格林-巴利综合征（GBS）的多神经性疾病。梭可引发GBS的空肠菌株携带wlaN或cgtB，编码产生唾液酸化脂寡糖（LOSSIAL）所需的β-1，3-半乳糖基转移酶的两种基因。我们描述了一个差异流行的基因wlaN和cgtB在C。空肠分离物来自三种不同的生态环境：人、肉鸡和野鸟。这两个基因的分布，这是相似的肉鸡和人类之间的分离株和不同的野生鸟类分离株相比，表明宿主依赖性的分布。此外，wlaN和cgtB基因的流行似乎仅限于某些克隆复合体。基因测序鉴定了wlaN基因内G-均聚物区的新变体的存在。此外，我们检测到两个变体的G丰富的区域内的cgtB基因，这表明，类似于wlaN，在cgtB基因的G-道介导的相位变化控制cgtB的表达。Caco-2细胞侵袭测定表明LOSSIAL的产生与侵袭真核细胞的能力之间没有明显的相关性。
Campylobacter jejuni causes campylobacteriosis, a bacterial gastroenteritis with high incidence worldwide. Moreover, C. jejuni infection can trigger the polyneuropathic disorder denominated Guillain-Barre syndrome (GBS). The C. jejuni strains that can elicit GBS carry either wlaN or cgtB, coding both genes for a beta-1,3-galactosyltransferase enzyme that is required for the production of sialylated lipooligosaccharide (LOSSIAL). We described a differential prevalence of the genes wlaN and cgtB in C. jejuni isolates from three different ecological niches: humans, broiler chickens, and wild birds. The distribution of both genes, which is similar between broiler chicken and human isolates and distinct when compared to the wild bird isolates, suggests a host-dependent distribution. Moreover, the prevalence of the wlaN and cgtB genes seems to be restricted to some clonal complexes. Gene sequencing identified the presence of new variants of the G- homopolymeric tract within the wlaN gene. Furthermore, we detected two variants of a G rich region within the cgtB gene, suggesting that, similarly to wlaN, the G-tract in the cgtB gene mediates the phase variation control of cgtB expression. Caco-2 cell invasion assays indicate that there is no evident correlation between the production of LOSSIAL and the ability to invade eukaryotic cells.