Enhancement of the mitogenic effect by artificial juxtacrine stimulation using immobilized EGF.
Enhancement of the mitogenic effect by artificial juxtacrine stimulation using immobilized EGF.
复制标题
使用固定化 EGF 进行人工近分泌刺激，增强促有丝分裂作用。
DOI：
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发表时间：
1997
期刊：
影响因子：
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通讯作者：
M. Kasuga
中科院分区：
文献类型：
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作者：
Y. Ito;J. Li;T. Takahashi;Y. Imanishi;Y. Okabayashi;Y. Kido;M. Kasuga
将小鼠表皮生长因子（EGF）与水溶性聚合物聚（丙烯酸）（EGF-PAA）或与水不溶性聚合物表面水解的聚（甲基丙烯酸甲酯）（EGF-PMMA）共价结合。固定化EGF（EGF-PMMA）刺激DNA合成在中国仓鼠卵巢细胞过度表达EGF受体的量是5至10%的游离EGF所需的可比效果。此外，EGF-PMMA的最大促有丝分裂作用大于未结合的EGF或EGF-PAA。EGF、EGF-PAA和EGF-PMMA诱导EGF受体的自磷酸化和促分裂原活化蛋白激酶的刺激。然而，尽管EGF-PMMA延迟了这些作用的发生，但它们的持续时间比EGF和EGF-PAA长得多。与EGF和EGF-PAA不同，EGF-PMMA从培养物中取出后与细胞无关，也不诱导受体内化。因此，用PMMA固定的EGF培养细胞代表了用于研究膜结合生长因子对细胞的“阿曲他克林”刺激的模型系统。
Mouse epidermal growth factor (EGF) was covalently conjugated with the water-soluble polymer, poly(acrylic acid) (EGF-PAA), or with the water-insoluble polymer, surface-hydrolyzed poly(methyl methacrylate) (EGF-PMMA). Immobilized EGF (EGF-PMMA) stimulated DNA synthesis in Chinese hamster ovary cells overexpressing EGF receptors in amounts that were 5 to 10% of those of free EGF required for comparable effects. In addition, the maximal mitogenic effect of EGF-PMMA was greater than that of unconjugated EGF or EGF-PAA. EGF, EGF-PAA, and EGF-PMMA induced the autophosphorylation of EGF receptors and the stimulation of mitogen-activated protein kinase. However, whereas the onset of these effects was delayed with EGF-PMMA, they persisted for much longer than those of EGF and EGF-PAA. Unlike EGF and EGF-PAA, EGF-PMMA was not associated with cells after their removal from culture and did not induce receptor internalization. Culturing cells with PMMA-immobilized EGF thus represents a model system for studying "juxtacrine" stimulation of cells by membrane-bound growth factors.