Hey genes:: a novel subfamily of hairy- and Enhancer of split related genes specifically expressed during mouse embryogenesis
Hey genes:: a novel subfamily of hairy- and Enhancer of split related genes specifically expressed during mouse embryogenesis
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DOI：
10.1016/s0925-4773(99)00080-5
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发表时间：
1999-07-01
影响因子：
2.6
通讯作者：
Gessler, M
中科院分区：
文献类型：
--
作者：
Leimeister, C;Externbrink, A;Gessler, M
我们已经确定了一个新的哺乳动物多毛/分裂增强子（E（spl））相关的碱性螺旋-环-螺旋（bHLH）基因的亚家族连同一个假定的果蝇同源。Hairy/E（spl）蛋白的特征在于在碱性结构域中具有不变的脯氨酸残基和羧基末端的groucho结合WRPW基序，而我们的基因编码羧基末端的KPG序列，因此被命名为Hev基因（Hairy/E（spl）-related with YRPW motif）。此外，它们具有独特的C-末端TE（I/V）GAF基序，并且在所有Hey家族成员中特征性脯氨酸改变为甘氨酸。RNA原位杂交分析显示Hey 1在神经系统、体节、心脏和颅面区域发育过程中特异性表达。Hey 2在体节中表达相似，而在心脏、颅面区域和神经系统中显示互补表达。表达模式的多样性意味着在神经发生、体节发生和器官发生中的独特功能。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
We have identified a novel subfamily of mammalian hairy/Enhancer of split (E(spl))-related basic helix-loop-helix (bHLH) genes together with a putative Drosophila homologue. While hairy/E(spl) proteins are characterized by an invariant proline residue in the basic domain and a carboxyterminal groucho-binding WRPW motif, our genes encode a carboxyterminal KPG sequence and were thus designated as Hev genes (Hairy/E(spl)-related with YRPW motif). Furthermore, they bear a unique C-terminal TE(I/V)GAF motif and the characteristic proline is changed in all Hey family members to glycine. RNA in situ hybridization analysis revealed specific expression of Hey1 during development of the nervous system, the somites, the heart and the craniofacial region. Hey2 is similarly expressed in the somites whereas it. shows a complementary expression in the heart, the craniofacial region and the nervous system. The diversity of expression patterns implies unique functions in neurogenesis, somitogenesis and organogenesis. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.