A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women and rhesus monkeys.
A potential molecular mechanism for regulating pre-mRNA splicing of implantation-related genes through unique uterine expression of splicing factor SC35 in women and rhesus monkeys.
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DOI：
10.1530/rep.0.1240209
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发表时间：
2002-08
期刊：
影响因子：
3.8
通讯作者：
G. Nie;A. Hampton;G. Fu;Y.-X. Liu;J. Findlay;L. Salamonsen
中科院分区：
文献类型：
--
作者：
G. Nie;A. Hampton;G. Fu;Y.-X. Liu;J. Findlay;L. Salamonsen
剪接因子SC 35是剪接体的重要组成部分，剪接体是从前mRNA中去除内含子以提供选择性剪接同种型的细胞装置。许多与子宫容受性发育和胚胎着床相关的蛋白质以同种型存在，其组织特异性表达可以通过选择性剪接来调节。SC35被确定为在小鼠妊娠早期在着床部位增加。然而，目前的研究表明，SC35是存在于人类和恒河猴子宫内膜，该蛋白质增加在分泌期的发情周期与增殖期相比，在这两种灵长类动物，它是目前在一个独特的模式内的上皮细胞和基质细胞的细胞核，以及在血管系统的细胞。免疫反应蛋白的强度和染色的SC 35细胞的比例随着发情周期的阶段而改变。一个非常精确的表达模式的SC 35（蛋白质和mRNA）在恒河猴胎盘形成早期。在植入部位之间的第24天和第35天的早期妊娠，SC 35强烈表达在滋养层细胞壳内的细胞滋养层，在合体滋养层细胞在细胞柱的外围和细胞滋养层和合体滋养层在浮动绒毛。在邻近的母体蜕膜中，SC 35的表达较弱。这些结果表明SC 35在受体子宫的准备、提供分泌蛋白以支持胚泡发育和滋养层入侵中发挥作用。
Splicing factor SC35 is an essential component of the spliceosome, the cellular apparatus that removes introns from pre-mRNA to provide alternatively spliced isoforms. Many proteins associated with development of uterine receptivity and embryo implantation are present as isoforms, the tissue-specific expression of which may be regulated through alternative splicing. SC35 was identified as being increased at implantation sites during early pregnancy in mice. However, the present study has demonstrated that SC35 is present in human and rhesus monkey endometrium, that the protein is increased during the secretory phase of the oestrous cycle compared with the proliferative phase in both these primates and that it is present in a distinct pattern within the nucleus of both epithelial and stromal cells, as well as in cells of the vasculature. Both the intensity of immunoreactive protein and the proportion of cells that stain for SC35 alter with the phase of the oestrous cycle. A very precise expression pattern of SC35 (both protein and mRNA) was seen during early placentation in rhesus monkeys. At implantation sites between day 24 and day 35 of early pregnancy, SC35 was expressed strongly in cytotrophoblasts within the trophoblastic shell, in syncytiotrophoblast at the periphery of the cell column and in both cytotrophoblast and syncytiotrophoblast in the floating villi. In the adjacent maternal decidua, expression of SC35 was weak. These results indicate a role for SC35 in preparation of a receptive uterus, in the provision of secreted proteins to support blastocyst development and in trophoblast invasion.