Production of erythropoietin-like activity by a murine erythroleukemia cell line.
Production of erythropoietin-like activity by a murine erythroleukemia cell line.
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鼠红白血病细胞系产生促红细胞生成素样活性。
DOI：
10.1073/pnas.80.20.6269
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发表时间：
1983
影响因子：
11.1
通讯作者：
B. Varet
中科院分区：
文献类型：
--
作者：
P. Tambourin;N. Casadevall;J. Choppin;C. Lacombe;J. Heard;S. Fichelson;F. Wendling;B. Varet
一种可移植的小鼠白血病，主要由生物克隆的Friend辅助病毒诱导，在受体ICFW小鼠中诱导红细胞增多症。从这种可移植白血病中体外建立了白血病细胞系IW.32。丁酸钠和血红素在这些白血病细胞中诱导红系分化，这已经在其他红系白血病细胞中得到证实。该细胞系的上清液不具有形成脾脏病灶的病毒活性。然而，它诱导59Fe在多红细胞小鼠体内的掺入，并使小鼠和人cfu-e在体外分化为红系菌落。因此，这些红细胞白血病细胞产生了一种具有促红细胞生成素所有生物学特性的因子。IW.32上清在体外的促红细胞生成素活性（相当于0.5-1国际单位(IU) /ml）高于体内（0.15-0.3 IU/ml）。这种促红细胞生成素样活性在100摄氏度下保持3分钟稳定，排除了病毒引起这些作用的可能性。初步研究表明，IW.32因子的生化特性与Connaught step 3促红细胞生成素非常相似，从而支持IW.32因子确实是一种促红细胞生成素的假设。
A transplantable murine leukemia, primarily induced by a biologically cloned Friend helper virus, was shown to induce polycythemia in recipient ICFW mice. A leukemia cell line (IW.32) was established in vitro from this transplantable leukemia. Sodium butyrate and hemin induced erythroid differentiation in these leukemia cells as has already been shown with other erythroleukemia cells. The supernatant of this cell line was devoid of spleen focus-forming virus activity. However, it induced the incorporation of 59Fe in polycythemic mice and the in vitro differentiation of murine and human cfu-e into erythroid colonies. Therefore, these erythroleukemia cells produced a factor with all the biological properties of erythropoietin. The erythropoietic activity of IW.32 supernatant was higher in vitro [equivalent to 0.5-1 international unit (IU) of erythropoietin per ml] than in vivo (0.15-0.3 IU/ml). This erythropoietin-like activity was stable at 100 degrees C for 3 min, which ruled out the possibility that a virus was responsible for these effects. Preliminary studies demonstrated that the biochemical properties of the IW.32 factor are strongly similar to those of Connaught step 3 erythropoietin, thus supporting the hypothesis that the IW.32 factor is indeed an erythropoietin.