Aldehyde Dehydrogenase ALDH3F1 involvements in flowering time regulation through histone acetylation modulation on FLC
Aldehyde Dehydrogenase ALDH3F1 involvements in flowering time regulation through histone acetylation modulation on FLC
复制标题
醛脱氢酶 ALDH3F1 通过组蛋白乙酰化调节 FLC 参与开花时间调节
DOI：
10.1111/jipb.12893
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发表时间：
2019
影响因子：
11.4
通讯作者：
Hu Honghong
中科院分区：
文献类型：
--
作者：
Xu Danyun;Liu Qing;Chen Gang;Yan Zhiqiang;Hu Honghong
开花时间调控是开花植物整个生命过程中最重要的过程之一，而开花基因C(FLOWERING Locus C，FLC)是开花时间的中枢抑制基因。然而，代谢醋酸盐水平是否会影响开花时间尚不清楚。在此，我们报道了乙醛脱氢酶ALDH3F1通过FLC依赖的途径在花的转变中起着重要的作用。在突变体dh3f1-1中，开花时间显著早于Col-0，FLC表达水平降低。ALDH3F1具有影响植物体内乙酸乙酯水平的乙醛脱氢酶活性，而E214和C252的氨基酸是其催化活性所必需的。此外，aldh3F1突变还降低了组蛋白H3上的乙酸酯水平和总乙酰化水平。在dh3f1-1中，FLC基因座上的H3K9Ac水平降低，从而降低了FLC的表达。ALDH3F1的表达可以挽救H3K9Ac在FLC和FLC上的表达，也可以挽救ALDH3F1-1的早花表型，而ALDH3F1E214Aa或ALDH3F1C252A则不能。我们的研究结果表明，ALDH3F1通过调节乙酰辅酶A乙酸酯的供应，作为组蛋白乙酰化供体来调节FLC基因上的H3K9Ac，从而参与了开花时间的调控。
Flowering time regulation is one of the most important processes in the whole life of flowering plants andFLOWERING LOCUS C(FLC) is a central repressor of flowering time. However, whether metabolic acetate level affects flowering time is unknown. Here we report that ALDEHYDE DEHYDROGENASE ALDH3F1 plays essential roles in floral transition viaFLC‐dependent pathway. In thealdh3f1‐1mutant, the flowering time was significant earlier than Col‐0 and theFLCexpression level was reduced. ALDH3F1 had aldehyde dehydrogenase activity to affect the acetate level in plants, and the amino acids of E214 and C252 are essential for its catalytic activity. Moreover,aldh3f1mutation reduced acetate level and the total acetylation on histone H3. The H3K9Ac level onFLClocus was decreased inaldh3f1‐1, which reducedFLCexpression. Expression ofALDH3F1could rescue the decreased H3K9Ac level onFLC, FLCexpression and also the early‐flowering phenotype ofaldh3f1‐1, howeverALDH3F1E214AorALDH3F1C252Acould not. Our findings demonstrate thatALDH3F1participates in flowering time regulation through modulating the supply of acetate for acetyl‐CoA, which functions as histone acetylation donor to modulate H3K9Ac onFLClocus.