Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12
Regulatory Role of PlaR (YiaJ) for Plant Utilization in Escherichia coli K-12
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DOI：
10.1038/s41598-019-56886-x
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发表时间：
2019-12-31
影响因子：
4.6
通讯作者：
Ishihama, Akira
中科院分区：
文献类型：
--
作者：
Shimada, Tomohiro;Yokoyama, Yui;Ishihama, Akira
在温血动物宿主之外，大肠杆菌K-12也能够在紧张的自然环境中生长和生存。自然界的主要有机物质是植物，但大肠杆菌的遗传系统如何利用植物来源的物质作为营养物质却知之甚少。在这里，我们使用gSELEX筛选系统描述了大肠杆菌基因组上未表征的iclr家族转录因子YiaJ的一组调控靶点。在总共18个高亲和力的YiaJ结合靶点中，鉴定出主要的调控靶点是yiaLMNOPQRS操纵子，用于利用水果中的抗坏血酸和植物果胶中的半乳糖酸。yaaj的靶标还包括参与利用其他植物源性物质作为营养物质的基因，如果糖、山梨醇、甘油和果糖赖氨酸。详细的体外和体内分析表明，l -抗坏血酸盐和α - d -半乳糖酸盐是调节YiaJ功能的效应配体。这些发现表明，YiaJ在一组基因的表达中起着主要的调节作用，这些基因用于利用植物来源的物质作为生存的营养物质。PlaR也被认为在自然应激环境下对大肠杆菌起保护作用，包括形成生物膜。然后我们建议将YiaJ重新命名为PlaR（植物利用调节剂）。肠杆菌大肠杆菌的天然宿主是温血动物，但即使在宿主之外，大肠杆菌也能在紧张的环境下存活。在地球上，大肠杆菌最常被用作营养物质的有机物质是植物源性成分，但迄今为止，人们对大肠杆菌用于植物利用的遗传系统知之甚少。在gSELEX筛选迄今尚未表征的tf调控靶点的过程中，我们在本研究中发现了iclr家族YiaJ参与了大约20个基因或操作子的调控，其中大多数与植物源物质如抗坏血酸、半乳糖酸、山梨醇、果糖和果糖酪氨酸的分解代谢有关。因此，我们建议将YiaJ重新命名为PlaR （regulator of plant utilization）。
Outside a warm-blooded animal host, the enterobacterium Escherichia coli K-12 is also able to grow and survive in stressful nature. The major organic substance in nature is plant, but the genetic system of E. coli how to utilize plant-derived materials as nutrients is poorly understood. Here we describe the set of regulatory targets for uncharacterized IclR-family transcription factor YiaJ on the E. coli genome, using gSELEX screening system. Among a total of 18 high-affinity binding targets of YiaJ, the major regulatory target was identified to be the yiaLMNOPQRS operon for utilization of ascorbate from fruits and galacturonate from plant pectin. The targets of YiaJ also include the genes involved in the utilization for other plant-derived materials as nutrients such as fructose, sorbitol, glycerol and fructoselysine. Detailed in vitro and in vivo analyses suggest that L-ascorbate and alpha-D-galacturonate are the effector ligands for regulation of YiaJ function. These findings altogether indicate that YiaJ plays a major regulatory role in expression of a set of the genes for the utilization of plant-derived materials as nutrients for survival. PlaR was also suggested to play protecting roles of E. coli under stressful environments in nature, including the formation of biofilm. We then propose renaming YiaJ to PlaR (regulator of plant utilization). The natural hosts of enterobacterium Escherichia coli are warm-blooded animals, but even outside hosts, E. coli can survive even under stressful environments. On earth, the most common organic materials to be used as nutrients by E. coli are plant-derived components, but up to the present time, the genetic system of E. coli for plant utilization is poorly understand. In the course of gSELEX screening of the regulatory targets for hitherto uncharacterized TFs, we identified in this study the involvement of the IclR-family YiaJ in the regulation of about 20 genes or operons, of which the majority are related to the catabolism of plant-derived materials such as ascorbate, galacturonate, sorbitol, fructose and fructoselysine. Therefore, we propose to rename YiaJ to PlaR (regulator of plant utilization).