Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species
Flow cytometric evidence for endopolyploidy in seedlings of some Brassica species
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DOI：
10.1007/s001220051624
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发表时间：
2001-01-01
影响因子：
5.4
通讯作者：
Kimura, Y
中科院分区：
文献类型：
--
作者：
Kudo, N;Kimura, Y
对甘蓝和甘蓝幼苗体细胞核 DNA 含量的流式细胞术分析显示，存在广泛的核内复制，导致组织中含有具有多个倍性水平的细胞（也称为“内多倍体”）。在白菜中观察到对应于 2C、4C、8C、16C、32C 和 64C 的单倍体核基因组补体 (1C) 的倍数：而甘蓝则表现出具有 5 个倍性水平（2C、4C、8C、16C 和 32C）的细胞混合物。具有不同倍性水平的细胞的分布具有组织特异性和发育阶段的特征。在干种子的胚中没有发现多倍体。幼苗发育过程中发生快速核内复制。最有可能的是，多倍性即使不是普遍特征，至少在芸苔属物种中也很常见。讨论了这一原始特征的生理和遗传意义。
Flow cytometric analysis of the nuclear DNA contents of somatic tissues from seedlings of Brassica rapa L. and B. oleracea L. revealed extensive endoreduplication, resulting in tissues that contain cells with multiple ploidy levels (also called 'endopolyploidy'). Multiples of the haploid nuclear genome complement (1C) corresponding to 2C, 4C, 8C, 16C, 32C and 64C were observed in Brassica rapa: while B. oleracea exhibited a mixture of cells with five ploidy levels, 2C, 4C, 8C, 16C and 32C. The distribution of cells with the different ploidy levels was tissue-specific and characteristic of the stage of development. Multiploidy was not found in the embryos of dry seeds. Rapid endoreduplication occurred during seedling development. It is most probable that multiploidy is, if not a general feature, at least very common in Brassica species. The physiological and genetic implications of this original feature are discussed.