DIRECT EVIDENCE FOR THE NONRANDOM LOCALIZATION OF MAMMALIAN CHROMOSOMES IN THE INTERPHASE NUCLEUS
DIRECT EVIDENCE FOR THE NONRANDOM LOCALIZATION OF MAMMALIAN CHROMOSOMES IN THE INTERPHASE NUCLEUS
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DOI：
10.1016/0014-4827(86)90199-0
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发表时间：
1986-11-01
影响因子：
3.7
通讯作者：
RINGERTZ, NR
中科院分区：
文献类型：
--
作者：
HADLACZKY, G;WENT, M;RINGERTZ, NR
用人抗着丝粒自身抗体间接免疫荧光染色方法，分析了大鼠-袋鼠(PTO)和印度仓鼠(IM)细胞间期核的染色体拓扑结构。在一些细胞中，着丝粒成对排列，表明同源染色体的联合。也观察到着丝粒聚集在核的一极(Rabl构型)和其他表明更高级别组织的图案。在五分之一的IM细胞中，可以根据免疫染色着丝粒的形态确定每一条染色体的核内位置。在可以识别着丝粒的IM细胞中，30%的同源染色体占据了间期染色质内的相邻区域。
Indirect immunofluorescence staining with human anti-centromere autoantibodies from a patient (LU 851) suffering from the CREST form of scleroderma was used to analyse chromosome topology in interphase nuclei of rat-kangaroo (PTO) and Indian muntjac (IM) cells. In some cells, centromeres were arranged in pairs suggesting association of homologous chromosomes. Clustering of centromeres at one pole of the nucleus (Rabl configuration) and other patterns suggesting higher order organization were also observed. In one fifth of the IM cells it was possible to identify the intranuclear location of each single chromosome on the basis of the morphology of the immunostained centromeres. In 30% of the IM cells in which centromeres could be identified, homologous chromosomes occupied adjacent territories within the interphase chromatin.