Three divisions of the mouse caudal striatum differ in the proportions of dopamine D1 and D2 receptor-expressing cells, distribution of dopaminergic axons, and composition of cholinergic and GABAergic interneurons
Three divisions of the mouse caudal striatum differ in the proportions of dopamine D1 and D2 receptor-expressing cells, distribution of dopaminergic axons, and composition of cholinergic and GABAergic interneurons
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DOI：
10.1007/s00429-019-01928-3
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发表时间：
2019-11-01
影响因子：
3.1
通讯作者：
Fukuda, Takaichi
中科院分区：
文献类型：
--
作者：
Miyamoto, Yuta;Nagayoshi, Issei;Fukuda, Takaichi
纹状体的大部分由纹状体和基质区室组成，但我们最近证明在小鼠尾侧纹状体的腹侧半部存在一个具有独特结构组织的区域（Miyamoto 等人，in Brain Struct Funct 223:4275-4291, 2018）。该区域根据其对 P 物质和脑啡肽的不同免疫反应性而被称为三层部分，由内侧、中间和外侧部分组成。在本研究中，我们使用免疫组织化学定量分析了每个分裂中投射神经元和中间神经元的分布。表达多巴胺 D1 受体 (D1R) 或 D2 受体 (D2R) 的两种类型的投射神经元在整个三层部分表现出互补分布，但三个部分之间的比例显着不同。内侧、中间和外侧分裂中表达 D1R 的神经元的比例分别为 88.6 +/- 8.2%（来自 3 只小鼠的 651 个细胞）、14.7 +/- 3.8%（1025 个细胞）和 49.3 +/- 4.5%（873 个细胞）。中间分裂的进一步特征是酪氨酸羟化酶免疫反应性轴突的神经支配较差。胆碱乙酰转移酶免疫反应性神经元的数量密度在三个分区中按照从内侧到外侧的顺序存在差异。相比之下，PV 阳性体细胞以恒定密度分布在所有三个区系中。标记一氧化氮合酶和钙结合蛋白的两种类型的 GABA 能中间神经元在内侧分裂中显示出最高的细胞密度。目前的结果将小鼠尾部纹状体的三个部分描述为不同的结构，这将有助于研究基底神经节中的新功能环。
The greater part of the striatum is composed of striosomes and matrix compartments, but we recently demonstrated the presence of a region that has a distinct structural organization in the ventral half of the mouse caudal striatum (Miyamoto et al. in Brain Struct Funct 223:4275-4291, 2018). This region, termed the tri-laminar part based upon its differential immunoreactivities for substance P and enkephalin, consists of medial, intermediate, and lateral divisions. In this study, we quantitatively analyzed the distributions of both projection neurons and interneurons in each division using immunohistochemistry. Two types of projection neurons expressing either the dopamine D1 receptor (D1R) or D2 receptor (D2R) showed complementary distributions throughout the tri-laminar part, but the proportions significantly differed among the three divisions. The proportion of D1R-expressing neurons in the medial, intermediate, and lateral divisions was 88.6 +/- 8.2% (651 cells from 3 mice), 14.7 +/- 3.8% (1025 cells), and 49.3 +/- 4.5% (873 cells), respectively. The intermediate division was further characterized by poor innervation of tyrosine hydroxylase immunoreactive axons. The numerical density of choline acetyltransferase immunoreactive neurons differed among the three divisions following the order from the medial to lateral divisions. In contrast, PV-positive somata were distributed throughout all three divisions at a constant density. Two types of GABAergic interneurons labeled for nitric oxide synthase and calretinin showed the highest cell density in the medial division. The present results characterize the three divisions of the mouse caudal striatum as distinct structures, which will facilitate studies of novel functional loops in the basal ganglia.