The Transcriptional Differences of Avian CD4(+)CD8(+) Double-Positive T Cells and CD8(+) T Cells From Peripheral Blood of ALV-J Infected Chickens Revealed by Smart-Seq2.
The Transcriptional Differences of Avian CD4(+)CD8(+) Double-Positive T Cells and CD8(+) T Cells From Peripheral Blood of ALV-J Infected Chickens Revealed by Smart-Seq2.
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Smart-Seq2揭示禽类CD4 CD8双阳性T细胞和ALV-J感染鸡外周血CD8 T细胞的转录差异
DOI：
10.3389/fcimb.2021.747094
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发表时间：
2021
影响因子：
5.7
通讯作者：
Liao M
中科院分区：
文献类型：
--
作者：
Dai M;Zhao L;Li Z;Li X;You B;Zhu S;Liao M
众所周知，鸡的CD8+ T细胞反应对清除病毒感染至关重要。然而，尚未比较鸡外周血单核细胞（PBMCs）中表达CD8受体的T细胞亚群之间的差异。本研究采用Smart-Seq2 scRNA-seq技术对禽白血病病毒J亚群（ALV-J）感染鸡PBMCs中CD8high+、CD8high αα+、CD8high αβ+、CD8medium+和CD4+CD8low+ T细胞亚群的差异进行了分析。加权基因共表达网络分析（WGCNA）和趋势分析显示，“细胞因子-细胞因子受体相互作用”途径富集的基因在CD8高αα+ T细胞群中表达量最高，尤其是CD40LG、IL2RA、IL2RB、IL17A、IL1R1、TNFRSF25和TNFRSF11等T细胞活化或应答相关基因，提示CD8高αα+ T细胞比其他CD8亚群对ALV-J感染的反应更强。另一方面，涉及“FoxO信号通路”和“tgf - β信号通路”的基因在CD4+CD8low+ (CD8low+) T细胞群中表达量最高，CD4+CD8low+ T细胞的功能可能通过CCND1、ROCK1、FOXO1、FOXO3、TNFRSF18、TNFRSF21的高表达而负调控T细胞的功能。经qRT-PCR确认的CD8+ T细胞和CD4+CD8low+双阳性T细胞中所选择的基因表达与Smart-Seq2数据吻合，说明smart-seq结果的可靠性。Granzyme K、Granzyme A、CCL5的高表达提示CD8+ T细胞的阳性反应。相反，CD4+CD8+ T细胞具有抑制活性可能是基于激活分子的低表达而T细胞活性抑制基因的高表达。这些发现证实了鸡pbmc中表达CD8受体的T细胞的异质性和转录差异。
It is well known that chicken CD8+ T cell response is vital to clearing viral infections. However, the differences between T cell subsets expressing CD8 receptors in chicken peripheral blood mononuclear cells (PBMCs) have not been compared. Herein, we used Smart-Seq2 scRNA-seq technology to characterize the difference of chicken CD8high+, CD8high αα+, CD8high αβ+, CD8medium+, and CD4+CD8low+ T cell subsets from PBMCs of avian leukosis virus subgroup J (ALV-J)-infected chickens. Weighted gene co-expression network analysis (WGCNA) and Trend analysis revealed that genes enriched in the “Cytokine–cytokine receptor interaction” pathway were most highly expressed in the CD8high αα+ T cell population, especially T cell activation or response-related genes including CD40LG, IL2RA, IL2RB, IL17A, IL1R1, TNFRSF25, and TNFRSF11, suggesting that CD8high αα+ T cells rather than other CD8 subpopulations were more responsive to ALV-J infections. On the other hand, genes involved in the “FoxO signaling pathway” and “TGF-beta signaling pathway” were most highly expressed in the CD4+CD8low+ (CD8low+) T cell population and the function of CD4+CD8low+ T cells may play roles in negatively regulating the functions of T cells based on the high expression of CCND1, ROCK1, FOXO1, FOXO3, TNFRSF18, and TNFRSF21. The selected gene expressions in CD8+ T cells and CD4+CD8low+ double-positive T cells confirmed by qRT-PCR matched the Smart-Seq2 data, indicating the reliability of the smart-seq results. The high expressions of Granzyme K, Granzyme A, and CCL5 indicated the positive response of CD8+ T cells. Conversely, CD4+CD8+ T cells may have the suppressor activity based on the low expression of activation molecules but high expression of T cell activity suppressor genes. These findings verified the heterogeneity and transcriptional differences of T cells expressing CD8 receptors in chicken PBMCs.
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WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
4
作者：
Bishop, Kathleen A.;Wang, Xiaohua;Coy, Heidi M.;Meyer, Mark B.;Gumperz, Jenny E.;Pike, J. Wesley
通讯作者：
Pike, J. Wesley
DOI：
10.1158/1078-0432.ccr-21-0573
发表时间：
2021-11-01
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lone W;Bouska A;Sharma S;Amador C;Saumyaranjan M;Herek TA;Heavican TB;Yu J;Lim ST;Ong CK;Slack GW;Savage KJ;Rosenwald A;Ott G;Cook JR;Feldman AL;Rimsza LM;McKeithan TW;Greiner TC;Weisenburger DD;Melle F;Motta G;Pileri S;Vose JM;Chan WC;Iqbal J
通讯作者：
Iqbal J
影响因子：
4.4
作者：
Sarkar, Surojit;Yuzefpolskiy, Yevgeniy;Kalia, Vandana
通讯作者：
Kalia, Vandana