Enzymatic modular assembly of hybrid Lewis antigens
Enzymatic modular assembly of hybrid Lewis antigens
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混合路易斯抗原的酶促模块化组装
DOI：
10.1039/d1ob01579f
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发表时间：
2021
影响因子：
3.2
通讯作者：
Chang-Cheng Liu
中科院分区：
文献类型：
--
作者：
Hui Xia;Jinfeng Ye;Hongzhi Cao;Xian-Wei Liu;Yan Zhang;Chang-Cheng Liu
混合Lewis抗原的酶促合成包括KH-1（Lewis y-Lewis x-Lactose、Ley-Lex-Lac）、Lewis a-Lewis x-Lactose（Lea-Lex-Lac）和Lewis b-Lewis x-Lactose（Leb-Lex-Lac）已通过简单的酶促模块化组装策略实现。从容易获得的四糖开始，使用 6 个酶模块，在少于 5 个连续酶促糖基化的线性步骤中获得了 3 种复杂的杂交 Lewis 抗原，总产率超过 40%。通过简单地控制供体-受体比率来实现区域选择性岩藻糖基化。该策略提供了一种在制备规模上轻松获得这些生物学上重要的复杂杂合路易斯抗原的方法。
The enzymatic synthesis of hybrid Lewis antigens including KH-1 (Lewis y-Lewis x-Lactose, Ley-Lex-Lac), Lewis a-Lewis x-Lactose (Lea-Lex-Lac), and Lewis b-Lewis x-Lactose (Leb-Lex-Lac) has been achieved using a facile enzymatic modular assembly strategy. Starting from a readily available tetrasaccharide, 3 complex hybrid Lewis antigens were achieved in over 40% total yields in less than 5 linear steps of sequential enzymatic glycosylation using 6 enzyme modules. The regio-selective fucosylation was achieved by simply controlling the donor–acceptor ratio. This strategy provides an easy access to these biologically important complex hybrid Lewis antigens at preparative scales.