Tumor-suppressive activity of Hnf1β in Wilms' tumor
Tumor-suppressive activity of Hnf1β in Wilms' tumor
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Hnf1 beta 在肾母细胞瘤中的肿瘤抑制活性
DOI：
10.1080/09168451.2019.1611409
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发表时间：
2019-05-02
影响因子：
1.6
通讯作者：
Xie, Yajun
中科院分区：
文献类型：
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作者：
Liu, Yamin;Kanyomse, Quist;Xie, Yajun
肝细胞核因子1β(HNF1β)是HNF-1家族的一种转录因子，与多种癌症有关，但其在肾母细胞瘤(肾母细胞瘤)中的作用尚未阐明。在这里，我们基于Oncomine数据库()，比较了它在Wilms‘s肿瘤患者肾组织和邻近组织中的表达。分析Wilms肿瘤来源的G401细胞的细胞增殖、凋亡、迁移和HNF1β表达水平。利用小鼠的各种组织(肺、心、肾等)，我们发现HNF1β在肾脏中表达最高。肿瘤学分析进一步证明，HNF1β在肾母细胞瘤组织中的表达低于癌旁组织。HNF1β过表达抑制细胞增殖和迁移，但促进细胞凋亡。敲除HNF1β基因产生了相反的结果。这些结果表明，HNF1β可能在肾发育和功能中起重要作用，其激活可能对肾母细胞瘤的发展起负性调节作用。
Hepatocyte nuclear factor 1 beta (HNF1 beta) is a transcription factor belonging to the HNF-1 family and has been implicated in a number of cancers, but its role in Wilms' tumor (nephroblastoma) has not been addressed. Here, we compared its expression between Wilms' tumor patient kidney tissue and adjacent tissue based on the Oncomine database (). Cell proliferation, apoptosis, migration, and HNF1 beta expression level were analyzed in Wilms' tumor-derived G401 cells. Using a variety of mouse tissues (lung, heart, kidney, etc.), we found that HNF1 beta is the highest expression in the kidneys. Oncomine analysis further demonstrated that HNF1 beta has a lower expression in Wilms' tumor tissue than in paracancerous tissues. Overexpression of HNF1 beta decreased cell proliferation and migration, but promoted cell apoptosis. Knockdown of HNF1 beta produced the opposite results. These results indicated that HNF1 beta may play important roles in kidney development and function, and its activation may negatively regulate Wilms' tumor progression.