Negative feedback loop formed by lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis
Negative feedback loop formed by lunatic fringe and Hes7 controls their oscillatory expression during somitogenesis
复制标题
DOI：
10.1002/gene.20171
复制
发表时间：
2005-12-01
期刊：
影响因子：
1.5
通讯作者：
Zhang, N
中科院分区：
文献类型：
--
作者：
Chen, J;Kang, L;Zhang, N
最近的研究表明，前体中胚层 (PSM) 中 Lfng 的循环表达在转录水平上受到 Notch 信号的控制，Notch 信号通过 CBF1 结合位点激活并周期性抑制 Lfng 启动子。在这里，我们提供了遗传证据，表明振荡表达是由负反馈机制控制的。我们还表明，另一种循环表达蛋白 Hes7 可以与 Ling 及其自身启动子上的 N 盒结合并抑制它们的活性。此外，我们证明 3' 非翻译区 (3'-UTR) 对于 Lfng mRNA 的快速降解很重要。
Recent studies show that the cyclic expression of Lfng in the presomitic mesoderm (PSM) is controlled at the transcription level by Notch signal through the CBF1 binding site for activation and periodic repression on the Lfng, promoter. Here we provide genetic evidence that the oscillatory expression is controlled by a negative feedback mechanism. We also show that Hes7, another cyclically expressed protein, can bind to the N-boxes on both Ling and its own promoters and repress their activity. In addition, we demonstrate that the 3' untranslated region (3'-UTR) is important for rapid degradation of Lfng mRNA.