Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight
Marker-assisted selection of two-line hybrid rice for disease resistance to rice blast and bacterial blight
复制标题
DOI：
10.1016/j.fcr.2015.07.018
复制
发表时间：
2015-12-01
影响因子：
5.8
通讯作者：
Li, Li
中科院分区：
文献类型：
--
作者：
Ni, Dahu;Song, Fengshun;Li, Li
稻瘟病和白叶枯病是世界主要稻区的严重病害。利用水稻抗性基因本身进行遗传育种将是对抗这些生物胁迫的经济且有效的方法。广展63S（GZ63S）是光周期热敏雄性不育（P/TGMS）优良系，衍生出许多在长江流域广泛栽培的两系超杂交品种。然而，GZ63S对稻瘟病和白叶枯病很敏感。在这里，我们通过分子标记辅助选择（MAS）报道了GZ63S及其衍生杂种良优6326中稻瘟病抗性基因Pi9和白叶枯病抗性基因Xa23的聚合。改良的不育系及其衍生的杂交种对所有八种测试的黄单胞菌 pv 表现出高水平的抗性。米酵母菌株。此外，它们在幼苗阶段表现出对叶瘟的高水平抗性，在成熟阶段对大多数自然地点的颈瘟表现出高水平的抗性。改良GZ63S及其衍生杂种在正常生长条件下保持了原品种的优良农艺性状。当暴露于疾病胁迫时，改良的杂交品种表现出比原始品种更好的农艺性状。我们的结果表明，通过 MAS 聚合 Pi9 和 Xa23 基因对于培育抗稻瘟病和白叶枯病的杂种很有用。 (C) 2015 Elsevier B.V. 保留所有权利。
Blast and bacterial blight are serious diseases around the major rice-growing area in the world. Genetic breeding by using rice resistance genes per se would be an economic and efficient way to combat these biotic stresses. Guangzhan63S (GZ63S) is an elite photoperiodic and thermo-sensitive male sterile (P/TGMS) line from which many widely cultivated two-line super-hybrid varieties in the Yangzi River basin have been derived. However, GZ63S is susceptible to rice blast as well as bacterial blight.Here, we report the pyramiding of the blast resistance gene Pi9 and the bacterial blight resistance gene Xa23 in GZ63S and its derived hybrid, Liangyou6326, by marker-assisted selection (MAS). The improved sterile line and its derived hybrid showed high levels of resistance to all eight tested Xanthomonas myzae pv. oryzae strains. Moreover, they demonstrated high levels of resistance to leaf blast at the seedling stage and to neck blast in most natural sites at the maturity stage. The improved GZ63S and its derived hybrid had maintained elite agronomic traits of original variety under normal growth conditions. When exposed to disease stresses, the improved hybrid represented much better agronomic traits than the original variety. Our results indicate that pyramiding the Pi9 and Xa23 genes by MAS is useful for breeding blast- and bacterial blight-resistant hybrids. (C) 2015 Elsevier B.V. All rights reserved.