Gut expression and regulation of FAT/CD36: possible role in fatty acid transport in rat enterocytes
Gut expression and regulation of FAT/CD36: possible role in fatty acid transport in rat enterocytes
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DOI：
10.1152/ajpendo.2001.281.5.e916
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发表时间：
2001-11-01
影响因子：
5.1
通讯作者：
Harmon, CM
中科院分区：
文献类型：
--
作者：
Chen, M;Yang, YK;Harmon, CM
脂肪酸转位酶（FAT）/CD 36是几种公认的质膜长链脂肪酸（LCFA）转运蛋白之一;然而，其在LCFA肠道吸收中的作用尚不清楚。我们假设FAT/CD 36在肠道发育过程中沿着肠长轴表达差异，提示其功能特异性。我们发现，肠粘膜FAT/CD 36 mRNA水平随沿着肠纵轴的解剖位置而变化：胃45 +/- 7，十二指肠173 +/- 29，空肠238 +/- 17，回肠117 +/- 14，结肠9 +/- 1%（平均值+/- SE，18 S mRNA作为对照）。FAT/CD 36蛋白水平在近端肠粘膜中也高于远端肠粘膜。粘液脂肪/CD 36 mRNA在肠成熟过程中也受到调节，从新生动物到成年动物增加了四倍。此外，脂肪/CD 36 mRNA水平和肠细胞LCFA摄取迅速下调，通过十二指肠内油酸输液。这些发现表明FAT/CD 36在小肠上皮细胞摄取LCFA中起作用。这可能对理解人体生理和病理生理条件下的脂肪酸吸收具有重要意义。
Fatty acid translocase (FAT)/CD36 is one of several putative plasma membrane long-chain fatty acid (LCFA) transport proteins; however, its role in intestinal absorption of LCFA is unknown. We hypothesized that FAT/CD36 would be differentially expressed along the longitudinal axis of the gut and during intestinal development, suggesting specificity of function. We found that intestinal mucosal FAT/CD36 mRNA levels varied by anatomic location along the longitudinal gut axis: stomach 45 +/- 7, duodenum 173 +/- 29, jejunum 238 +/- 17, ileum 117 +/- 14, and colon 9 +/- 1% (means +/- SE with 18S mRNA as control). FAT/CD36 protein levels were also higher in proximal compared with distal intestinal mucosa. Mucosal FAT/CD36 mRNA was also regulated during intestinal maturation, with a fourfold increase from neonatal to adult animals. In addition, FAT/CD36 mRNA levels and enterocyte LCFA uptake were rapidly downregulated by intraduodenal oleate infusion. These findings suggest that FAT/CD36 plays a role in the uptake of LCFA by small intestinal enterocytes. This may have important implications in understanding fatty acid absorption in human physiological and pathophysiological conditions.