Candida glabrata displays pseudohyphal growth
Candida glabrata displays pseudohyphal growth
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DOI：
10.1111/j.1574-6968.2000.tb09216.x
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发表时间：
2000-08-01
影响因子：
2.1
通讯作者：
Haynes, K
中科院分区：
文献类型：
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作者：
Csank, C;Haynes, K
据报道，经历形态变化的能力是真菌发病机制中的一个有利特征。在这里，我们证明光滑念珠菌 ATCC2001 与二倍体酿酒酵母菌株一样，在固体氮饥饿培养基 (SLAD) 上形成假菌丝细胞的细长链。相邻细胞之间有明显的收缩，没有看到平行边的菌丝，并且假菌丝侵入琼脂。当 SLAD 补充硫酸铵时，光滑念珠菌和二倍体酿酒酵母菌株都失去了假菌丝生长的能力。然而，在这种培养基上，光滑酵母细胞侵入琼脂的方式与在丰富的培养基（例如YPD）上培养的酿酒酵母单倍体菌株所表现出的侵入生长模式类似。 C. glabrata 无法侵入 YPD，这表明这两种真菌的丝化过程是不同的。据我们所知，这是第一份证明光滑 C. glabrata 可以发生形态变化并作为侵入性丝状生物生长的报告。 (C) 2000 年欧洲微生物学会联合会。由 Elsevier Science B.V. 出版。保留所有权利。
The ability to undergo morphological change has been reported as an advantageous trait in fungal pathogenesis. Here we demonstrate that Candida glabrata ATCC2001, like diploid Saccharomyces cerevisiae strains, forms elongated chains of pseudohyphal cells on solid nitrogen starvation media (SLAD). Constrictions were apparent between adjoining cells, no parallel-sided hyphae were seen and pseudohyphae invaded the agar. When SLAD was supplemented with ammonium sulfate both C. glabrata and diploid S. cerevisiae strains lost their ability to undergo pseudohyphal growth. However, on this media C. glabrata yeast cells invaded the agar in a similar fashion to the invasive growth mode exhibited by haploid strains of S. cerevisiae cultured on rich media such as YPD. C. glabrata was not capable of invading YPD demonstrating that the process of filamentation is distinct in these two fungi. To our knowledge this is the first report to demonstrate that C. glabrata can undergo morphological change and grow as an invasive filamentous organism. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.