Regulated production of mu m and mu s mRNA requires linkage of the poly(A) addition sites and is dependent on the length of the mu s-mu m intron.
Regulated production of mu m and mu s mRNA requires linkage of the poly(A) addition sites and is dependent on the length of the mu s-mu m intron.
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mu m 和 mu s mRNA 的调节生产需要连接 Poly(A) 添加位点，并且取决于 mu s-mu m 内含子的长度。
DOI：
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发表时间：
1986
影响因子：
11.1
通讯作者：
R. Perry
中科院分区：
文献类型：
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作者：
M. Peterson;R. Perry
编码MU重链的膜相关形式(MUm)和分泌型(Mu S)的mRNAs是由同一免疫球蛋白基因的转录本通过差异RNA加工而来的。为了帮助阐明在B淋巴样细胞成熟过程中调节这两个mumRNAs的产生的机制，我们制备了一系列特异性修饰的mu链基因，并研究了它们在转染代表发育早期和发育晚期的细胞时的表达。我们已经确定，适当的调控取决于亩S和亩m聚(A)添加位点的连锁以及亩S-亩m内含子的长度。从这个内含子的中心区删除一个800到900个核苷酸的片段可以取消调控；用各种DNA序列替换这个片段可以恢复它。根据这些结果，我们提出了一个模型，在该模型中，调控主要是通过Mu-S位点的切割/多腺苷基化与C-Mu-4和Mu-m外显子的剪接之间的竞争来实现的。
mRNAs encoding the membrane-associated (mu m) and secreted (mu s) forms of mu heavy chain are derived from transcripts of the same immunoglobulin gene by differential RNA processing. To help elucidate the mechanism that regulates the production of these two mu mRNAs during the course of B-lymphoid maturation, we produced a series of specifically modified mu-chain genes and studied their expression when transfected into cells representing either early or late developmental stages. We have established that proper regulation depends on linkage of the mu s and mu m poly(A) addition sites and the length of the mu s-mu m intron. Deletion of an 800 to 900-nucleotide segment from the central region of this intron abolishes regulation; replacement of this segment with miscellaneous DNA sequences restores it. From these results we propose a model in which regulation is principally achieved by competition between cleavage/polyadenylylation of the mu s site and splicing of the C mu 4 and mu m exons.