Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved by treatment with excessive MgCl2
Enhancement of transglutaminase production in Streptomyces mobaraensis as achieved by treatment with excessive MgCl2
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通过过量 MgCl2 处理增强茂原链霉菌的转谷氨酰胺酶产量
DOI：
10.1007/s00253-011-3790-5
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发表时间：
2012-03-01
影响因子：
5
通讯作者：
Zhang, Yanhe
中科院分区：
文献类型：
--
作者：
Zhang, Lili;Zhang, Lanwei;Zhang, Yanhe
在这项研究中，我们首先测试了八种不同的盐作为生产转氨酶（TGase）的应激介导的生物过程的能力。当基础培养基补充0.10 M MgCl 2时，在孵育96 h时观察到TGase的最高产量（4.3 U/ml），这与基础培养基对照（120 h时为2.1 U/ml）观察到的结果相反。Western印迹分析的数据表明，在MgCl 2培养基中，pro-TGase转化为成熟酶的速度更快。此外，总蛋白酶，金属蛋白酶，丝氨酸蛋白酶也以更快的速度合成在培养基中含有氯化镁。结果表明，MgCl 2增强了参与TGase生物合成活化的关键蛋白酶的产生。为了探讨其机制，进行了活力测定。结果表明，MgCl 2诱导菌丝分化，降低细胞生长速率，促进细胞死亡。我们认为，TGase的生产是由MgCl 2胁迫诱导的菌丝体分化的刺激促进。
In this study, we first tested the capacity for eight different salts as stress-mediated bioprocesses in the production of transglutaminase (TGase). A significant effect on the cell growth and TGase production was obtained with the highest yield of TGase being observed at 96 h of incubation (4.3 U/ml) when the basic medium was supplemented 0.10 M MgCl2, as opposed to that observed with the basic medium control (2.1 U/ml at 120 h). Data from Western blot assays showed that transformation of pro-TGase to its mature enzyme occurred more rapidly in MgCl2 medium. Furthermore, total protease, metalloprotease, and serine protease were also synthesized at a faster rate in the medium containing MgCl2. The results demonstrate that MgCl2 enhanced the production of key proteases involved in the activation of TGase biosynthesis. To explore the mechanism, viability assay was performed. The results show that MgCl2 induced the mycelia differentiation, decreased cell growth rate, and stimulated cell death. We argue that TGase production was promoted by the stimulation of mycelium differentiation induced by MgCl2 stress.