Early post-stressor intervention with minocycline, a second-generation tetracycline, attenuates post-traumatic stress response in an animal model of PTSD
Early post-stressor intervention with minocycline, a second-generation tetracycline, attenuates post-traumatic stress response in an animal model of PTSD
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DOI：
10.1016/j.euroneuro.2014.11.012
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发表时间：
2015-01-01
影响因子：
5.6
通讯作者：
Cohen, Hagit
中科院分区：
文献类型：
--
作者：
Levkovitz, Yechiel;Fenchel, Daphna;Cohen, Hagit
我们在创伤后应激障碍（PTSD）动物模型中评估了米诺环素（一种具有抗炎、抗细胞凋亡和神经保护能力的四环素）的作用。大鼠暴露于心因性应激，1h后给予二甲胺四环素或生理盐水处理。行为学测量包括应激暴露后7 d的升高+迷宫（EPM）和声惊吓反应（ASR）。行为测试后一天，动物暴露于创伤提示和冻结反应评估。然后检测海马、额叶皮质（FC）和下丘脑局部细胞因子白介素-1 （IL-1）、白介素-6 （IL-6）和肿瘤坏死因子-a （tnf - α）水平。二甲胺四环素减轻应激暴露大鼠的焦虑样行为。此外，与用生理盐水处理的大鼠相比，用二甲胺四环素处理的暴露大鼠的细胞因子水平下降。这项研究提示二甲胺四环素在预防急性暴露于心理应激引起的生理和行为改变方面的潜在应用。由于这是第一个在创伤后应激障碍动物模型中报告二甲胺四环素治疗有益结果的研究，因此需要进一步研究二甲胺四环素在应激相关疾病中的应用，重点是PTSD。(C) 2014 Elsevier B.V.和ECNP。版权所有。
We assessed the effects of minocycline, a tetracycline with anti-inflammatory, anti-apoptotic and neuroprotective capacities, in an animal model of post-traumatic stress disorder (PTSD). Rats were exposed to psychogenic stress and treated 1 h later with minocycline or saline. Behavioral measures included the elevated plus-maze (EPM) and acoustic startle response (ASR) 7 days post stress-exposure. One day after behavioral testing, animals were exposed to a trauma cue and freezing response was assessed. Local levels of cytokines interleukin-1 (IL-1), interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-alpha) in the hippocampus, frontal cortex (FC) and hypothalamus were then examined. Minocycline attenuated anxious-like behaviors in stress-exposed rats. In addition, decreased levels of cytokines were measured in exposed rats treated with minocycline compared to their counterparts treated with saline. This study suggests a potential use of minocycline in preventing physiological and behavioral alternations resulting from acute exposure to psychological stress. As this is the first study to report beneficial outcomes for minocycline treatment in an animal model of PTSD, further investigations of the use of minocycline in stress-related conditions with emphasis on PTSD is needed. (C) 2014 Elsevier B.V. and ECNP. All rights reserved.