Prostaglandin and leukotriene production by alveolar type II cells in vitro.
Prostaglandin and leukotriene production by alveolar type II cells in vitro.
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II 型肺泡细胞在体外产生前列腺素和白三烯。
DOI：
10.1152/ajplung.1990.258.4.l179
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Voelkel,NF
中科院分区：
文献类型：
--
作者：
Cott,GR;Westcott,JY;Voelkel,NF
从成年大鼠中分离肺泡 II 型细胞，培养 22 小时，并通过免疫测定对来自未刺激和刺激细胞的培养基中的个体类二十烷酸进行定量。钙离子载体 A23187 的刺激显着增加了前列腺素的介质水平（前列腺素和 6-酮前列腺素 F1 α 高于血栓素 B2）。与之前的报道相反，用 A23187 孵育的细胞中也恢复了白三烯培养基水平的增加，但仅限于培养时间小于或等于 24 小时的细胞。通过高效液相色谱和分光光度分析的组合证实了白三烯 C4 的产生。 II 型肺泡细胞培养物产生的类二十烷酸特征与类似培养的肺泡巨噬细胞明显不同。最后，用佛波酯或磷脂酶 C 刺激肺泡 II 型细胞培养物增加了培养基前列腺素水平，但未能增加白三烯水平。我们得出结论，肺泡 II 型细胞的原代培养物能够将花生四烯酸从头代谢为环氧合酶和脂氧合酶产物，并且白三烯的产生取决于培养时间和激动剂。因此，II 型肺泡细胞是体内产生这些类二十烷酸的潜在来源，并且产生的特定脂质介质可能根据病理生理刺激而变化。
Alveolar type II cells were isolated from adult rats, cultured for 22 h, and individual eicosanoids in the media from unstimulated and stimulated cells were quantified by immunoassay. Stimulation with the calcium ionophore A23187 significantly increased the media levels of prostaglandins (prostaglandin and 6-keto-prostaglandin F1 alpha greater than thromboxane B2). In contrast to previous reports, increased media levels of leukotrienes were also recovered from cells incubated with A23187, but only for cells in culture for less than or equal to 24 h. The production of leukotriene C4 was confirmed by a combination of high-performance liquid chromatography and spectrophotometric analysis. The profile of eicosanoids produced by cultures of alveolar type II cells was distinctly different than that of similarly cultured alveolar macrophages. Finally, stimulation of alveolar type II cell cultures with either a phorbol ester or phospholipase C increased media prostaglandin levels but failed to increase leukotriene levels. We conclude that primary cultures of alveolar type II cells are capable of the de novo metabolism of arachidonic acid to both cyclooxygenase and lipoxygenase products and that the production of leukotrienes is dependent on both time in culture and agonist. Thus alveolar type II cells are a potential source for the production of these eicosanoids in vivo, and the particular lipid mediators produced may vary depending on the pathophysiologic stimulus.