Applications of DNA tiling arrays to experimental genome annotation and regulatory pathway discovery
Applications of DNA tiling arrays to experimental genome annotation and regulatory pathway discovery
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DOI：
10.1007/s10577-005-2165-0
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Snyder, M
中科院分区：
文献类型：
--
作者：
Bertone, P;Gerstein, M;Snyder, M
微阵列已成为一种流行的和重要的技术调查全球模式的基因表达和调控。许多创新的实验已经将微阵列的应用扩展到mRNA表达水平的测量之外，以揭示大规模染色体功能和动力学的各个方面。由于最近平铺阵列的发展，这已经成为可能，其中包含染色体或基因座的所有非重复DNA以各种序列分辨率表示。由于平铺阵列被设计为包含整个DNA序列而无需事先咨询现有的基因注释，因此它们能够通过基因组基因座的无偏询问来发现新的转录序列和调控元件。实施这种方法来对大的真核生物基因组进行全局分析提出了重大的技术挑战。尽管如此，平铺阵列有望成为全基因组识别和功能元件的表征工具。结合计算方法来关联这些数据并绘制转录调节因子的复杂相互作用，平铺阵列实验可以提供对基本分子和细胞过程的更全面理解的见解。
Microarrays have become a popular and important technology for surveying global patterns in gene expression and regulation. A number of innovative experiments have extended microarray applications beyond the measurement of mRNA expression levels, in order to uncover aspects of large-scale chromosome function and dynamics. This has been made possible due to the recent development of tiling arrays, where all non-repetitive DNA comprising a chromosome or locus is represented at various sequence resolutions. Since tiling arrays are designed to contain the entire DNA sequence without prior consultation of existing gene annotation, they enable the discovery of novel transcribed sequences and regulatory elements through the unbiased interrogation of genomic loci. The implementation of such methods for the global analysis of large eukaryotic genomes presents significant technical challenges. Nonetheless, tiling arrays are expected to become instrumental for the genome-wide identification and characterization of functional elements. Combined with computational methods to relate these data and map the complex interactions of transcriptional regulators, tiling array experiments can provide insight toward a more comprehensive understanding of fundamental molecular and cellular processes.