Accumulation of adrenocorticotropin secretory granules in the midbody of telophase AtT20 cells: evidence that secretory granules move anterogradely along microtubules.
Accumulation of adrenocorticotropin secretory granules in the midbody of telophase AtT20 cells: evidence that secretory granules move anterogradely along microtubules.
复制标题
末期末期ATT20细胞中体中肾上腺皮质激素分泌颗粒的积累：分泌颗粒沿微管行进的证据。
DOI：
10.1083/jcb.104.4.1047
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发表时间：
1987-04
影响因子：
7.8
通讯作者：
Burke, B
中科院分区：
文献类型：
--
作者：
Tooze, J;Burke, B
在细胞周期中的ACTH分泌颗粒在AtT20细胞的分布，如所揭示的免疫荧光标记和电子显微镜的薄切片，经历了一个周期的变化。在间期细胞中，颗粒集中在高尔基体区域，在那里它们形成，并且也在细胞突起的尖端，在那里它们积累。这些突起包含许多延伸到其尖端的微管。在中期和后期，颗粒随机分布在圆形有丝分裂细胞的细胞质中。在进入末期时，有一个快速和显着的颗粒重新分布，在胞质分裂过程中，随着中间体的发育，这些颗粒在中间体中大量积累。这种分泌颗粒在中间体的积聚依赖于微管的存在。在细胞周期中的分泌颗粒的分布的变化模式实现了一个模型的预测，设想第一，分泌颗粒关联和移动沿着间期微管在一个净顺行方向远离中心粒，其次，他们不与微管的有丝分裂纺锤体在中期和后期。
During the cell cycle the distribution of the ACTH-containing secretory granules in AtT20 cells, as revealed by immunofluorescence labeling and electron microscopy of thin sections, undergoes a cycle of changes. In interphase cells the granules are concentrated in the Golgi region, where they form, and also at the tips of projections from the cells, where they accumulate. These projections contain many microtubules extending to their tips. During metaphase and anaphase the granules are randomly distributed in the cytoplasm of the rounded-up mitotic cells. On entry into telophase there is a rapid and striking redistribution of the granules, which accumulate in large numbers in the midbody as it develops during cytokinesis. This accumulation of secretory granules in the midbody is dependent upon the presence of microtubules. The changing pattern of distribution of the secretory granules during the cell cycle fulfills the predictions of a model envisaging first that secretory granules associate with and move along interphase microtubules in a net anterograde direction away from the centrioles, and secondly that they do not associate with microtubules of the mitotic spindle during metaphase and anaphase.