Association of interleukin-10 A-592C and T-819C polymorphisms with type 2 diabetes mellitus
Association of interleukin-10 A-592C and T-819C polymorphisms with type 2 diabetes mellitus
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DOI：
10.1016/j.humimm.2005.05.001
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发表时间：
2005-12-01
期刊：
影响因子：
2.7
通讯作者：
Shiau, Ming-Yuh
中科院分区：
文献类型：
--
作者：
Chang, Yih-Hsin;Huang, Chien-Ning;Shiau, Ming-Yuh
2型糖尿病（T2 DM）是糖尿病最常见的形式。最近确定了低白细胞介素（IL）-10产生能力与代谢综合征和T2 DM的相关性。人类IL-10分泌能力的大约75176的变化来自于导致疾病易感性的遗传因素。基于IL-10的分泌能力在转录水平受到严格控制以及IL-10的低产生能力与T2 DM相关的事实，研究IL-10基因启动子的多态性是否与T2 DM相关似乎是诱人的。采用聚合酶链反应-限制性片段长度多态性技术，对中山医科大学附属医院内科就诊的370例T2 DM患者进行IL-10基因启动子-592和-819位点多态性检测。尽管A-592 C和A-592 C之间没有显著的关联，（p = 0.088）或T-819 C（p = 0.160）多态性与T2 DM的相关性，显著更多的T2 DM受试者携带-592*C（34.28%，p = 0.027）和-819*C（32.57%，p < 0.001）等位基因，其与高水平的IL-10产生相关。然而，这两个多态性与T2 DM的生化标志物之间没有观察到关联。我们的研究表明，IL-10基因多态性可能在决定糖尿病易感性方面发挥特定作用，但在糖尿病的临床表现中似乎并不重要。
Type 2 diabetes mellitus (T2DM) is the most common form of diabetes. The association of low interleukin (IL)-10 production capacity with the metabolic syndrome and T2DM was recently established. Approximately 75176 of the variation in IL-10 secretion capacity in humans derives from genetic factors that contribute to disease susceptibility. Based on the facts that IL-10 secretion ability is tightly controlled at the transcription level and the low production capacity of IL-10 is associated with T2DM, it seemed tempting to investigate if polymorphisms in the IL-10 gene promoter contribute to T2DM. IL-10 promoter polymorphisms at positions -592 and -819 among 370 consecutive patients with T2DM seen at the Department of Internal Medicine, Chung Shan Medical University Hospital, were examined using polymerase chain reaction restriction fragment length polymorphism. Though no significant association was detected between either the A-592C (p = 0.088) or T-819C (p = 0.160) polymorphism and T2DM, significantly more T2DM subjects carried -592*C (34.28%, p = 0.027) and -819*C (32.57%, p < 0.001) alleles, which were associated with high levels of IL-10 production. Nevertheless, no association was observed between these two polymorphisms and biochemical markers for T2DM. Our study suggests that IL-10 genetic polymorphisms may play a specific role(s) in determining diabetic susceptibility, but do not seem to be important in the clinical manifestations of diabetes.