Electrochemically fabricated polyaniline nanowire-modified electrode for voltammetric detection of DNA hybridization
Electrochemically fabricated polyaniline nanowire-modified electrode for voltammetric detection of DNA hybridization
复制标题
DOI：
10.1016/j.electacta.2005.10.038
复制
发表时间：
2006-05
影响因子：
6.6
通讯作者：
Ningning Zhu;Zhu Chang;P. He;Yu-zhi Fang
中科院分区：
文献类型：
--
作者：
Ningning Zhu;Zhu Chang;P. He;Yu-zhi Fang
基于电化学方法制备的聚苯胺纳米线和亚甲基蓝构建了一种新型的DNA杂交检测电化学生物传感器。以玻碳电极（GCE）为工作电极，在苯胺电解质溶液中，通过三步电化学沉积法直接合成了导电聚合物纳米线。用场发射扫描电子显微镜（SEM）观察了聚苯胺薄膜的形貌。纳米线的直径在80 - 100 nm之间。聚苯胺修饰电极具有良好的电化学导电性能。5′端带有磷酸基团的寡核苷酸共价连接到电极上聚苯胺纳米线的氨基上。用亚甲基蓝（MB）作为指示剂，用差示脉冲伏安法（DPV）测量监测杂交事件。该方法可以有效地区分互补和非互补DNA序列，检测限为1.0×10 - 12 moll-1的互补靶，表明聚苯胺纳米线在灵敏的电化学生物传感器应用中具有很大的前景。
A novel and sensitive electrochemical DNA biosensor based on electrochemically fabricated polyaniline nanowire and methylene blue for DNA hybridization detection is presented. Nanowires of conducting polymers were directly synthesized through a three-step electrochemical deposition procedure in an aniline-containing electrolyte solution, by using the glassy carbon electrode (GCE) as the working electrode. The morphology of the polyaniline films was examined using a field emission scanning electron microscope (SEM). The diameters of the nanowires range from 80 to 100nm. The polyaniline nanowires-coated electrode exhibited very good electrochemical conductivity. Oligonucleotides with phosphate groups at the 5′ end were covalently linked onto the amino groups of polyaniline nanowires on the electrode. The hybridization events were monitored with differential pulse voltammetry (DPV) measurement using methylene blue (MB) as an indicator. The approach described here can effectively discriminate complementary from non-complementary DNA sequence, with a detection limit of 1.0×10−12moll−1of complementary target, suggesting that the polyaniline nanowires hold great promises for sensitive electrochemical biosensor applications.